Genome-wide Analysis of SBP-box Gene Family and Molecular Cloning of Its Typical Members in Malus × domestica

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (2): 215-226.

Genome-wide Analysis of SBP-box Gene Family and Molecular Cloning of Its Typical Members in Malus × domestica

ZHANG Xiao-hui1,*，WEI Xiao-chun1，LI Xi-xiang1，SUN Yu-yan1，WANG Guan1，CHANG Zhao-jing1，LIU Guan-qun1，QIU Yang1，SONG Jiang-ping1，WANG Hai-ping1，SHEN Di1，WANG Da-jiang2，and HAN Yue-peng3

（1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences；Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture，Beijing 100081，China；2Research Institute of Pomology，Chinese Academy of Agricultural Sciences，Xingcheng，Liaoning 125100，China；3Wuhan Botanical Garden，Chinese Academy of Sciences，Wuhan 430074，China）

Received:2013-09-24 Online:2014-02-25 Published:2014-02-25

Abstract

Abstract: The present study isolated 33 SBP-box family genes including 20 miR156 targets in Golden Delicious apple genome. The phylogenic analyses were performed and the biological functions were predicted based on the phylogenic tree. The existence and expression pattern of SBP-box family genes were investigated by PCR and RT-PCR analysis. Alternative splicing events were commonly found in this gene family. Four typical members，MdSPL2，MdSPL11，MdSPL13 and MdSPL33 were cloned from Fuji apple tree. Compared to Golden Delicious cultivar，MdSPL33 from Fuji produced an alternative splicing version which lost the 2nd and 3rd exons and harboring two single base mutations. MdSPL11 and MdSPL13 have four and two single base mutations causing three and one amino acid changes，respectively.

Key words: Malus×domestica, SBP-box family, phylogenetic, gene cloning

CLC Number:

S 661.1

Zhang-Xiao-Hui, WEI Xiao-Chun, LI Xi-Xiang, SUN Yu-Yan-1, WANG , GUAN 1, CHANG Zhao-Jing-1, LIU Guan-Qun-1, QIU , YANG 1, SONG Jiang-Ping-1, WANG Hai-Ping-1, SHEN , DI 1, WANG Da-Jiang-2, HAN Yue-Peng-3. Genome-wide Analysis of SBP-box Gene Family and Molecular Cloning of Its Typical Members in Malus × domestica[J]. ACTA HORTICULTURAE SINICA, 2014, 41(2): 215-226.

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Genome-wide Analysis of SBP-box Gene Family and Molecular Cloning of Its Typical Members in Malus × domestica

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