Abstract: 17 cucumber chromosome segment introgression lines（CSILs）were constructed from a cross between the powdery mildew susceptible cultivar（D8）and resistant cultivar（JIN5-508），and 63 plants with high powdery mildew resistance（disease index ≤ 10）were selected. In this study，324 pairs of SSR primers were applied to analysis the polymorphic level between JIN5-508 and D8，and 91 pairs of SSR primer which generated polymorphic bands were applied to analysis the distribution，segment size of the chromosome segments. The results indicated that 25 CSILs were single segment introgression line，which accounted for 39.68% of the total CSILs；Most of the CSILs were double segment introgression lines，take up to 50.79%；Four CSILs were imported 3 chromosome segments；No donor introgression segments were found in 2 lines. 4 donor segments occupied the highest frequency，which located on 23.4–41.3 cM of chromosome 1，51.1–54.0 cM of chromosome 3，9.3–11.3 cM of chromosome 4 and 1.4–3.2 cM of chromosome 6，respectively.

Key words: cucumber, powdery mildew, SSR, CSIL