The Character of MhHSP70 from Malus hupehensis and Its Response to the Stress of Cadmium and Osmosis

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (3): 417-424.

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The Character of MhHSP70 from Malus hupehensis and Its Response to the Stress of Cadmium and Osmosis

SHEN Wei，YANG Hong-qiang^*，LI Qiang，RAN Kun，JIANG Qian-qian，SUN Xiao-li，ZHANG Wei-wei，and SUI Jing

College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，National Research Center for Apple Engineering and Technology，Tai’an，Shandong 271018，China

Received:2011-12-26 Revised:2012-03-02 Online:2012-03-25 Published:2012-03-25
Contact: YANG Hong-qiang E-mail:hqyang@sdau.edu.cn；labft@sdau.edu.cn

Abstract

Abstract: The cDNA sequence of HSP70 gene，designated as MhHSP70（GenBank number： HQ876864），was isolated from Malus hupehensis（Pamp）Rehd. var. pinyiensis Jiang by reverse transcription polymerase chain reaction（RT-PCR）and rapid-amplification of cDNA ends（RACE）. The full length cDNA sequence was 2 307 bp，encoding a protein of 650 amino acids，which have close relationship with HSP70 from Malus pumila Mill.，Solanum lycopersicum and Nicotiana tabacum. The expression of MhHSP70 was induced by cadmium and osmotic stress in roots and leaves，the level of MhHSP70 expression was enhanced with the increase of NaCl and cadmium concentration，as well as PEG treatment time.

Key words: Malus hupehensis（Pamp）Rehd., MhHSP70；heat-shock protein, cadmium, osmosis, stress

SHEN Wei, YANG Hong-Qiang, LI Qiang, RAN Kun, JIANG Qian-Qian, SUN Xiao-Li, ZHANG Wei-Wei, SUI Jing. The Character of MhHSP70 from Malus hupehensis and Its Response to the Stress of Cadmium and Osmosis[J]. ACTA HORTICULTURAE SINICA, 2012, 39(3): 417-424.

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The Character of MhHSP70 from Malus hupehensis and Its Response to the Stress of Cadmium and Osmosis

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