Cloning and Expression of Flavonoid O-methyltransferase Gene in Ginkgo biloba

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (2): 355-362.

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Cloning and Expression of Flavonoid O-methyltransferase Gene in Ginkgo biloba

ZHANG Chuan-li¹，CHEN Peng^2,*，ZHONG Yue-ming²，ZHOU Chang-yuan²，LIANG Guo-hua³，SHEN Dan-hong²，and WU Qiu-yue²

¹ College of Bioscience and Biotechnology，Yangzhou University，Yangzhou，Jiangsu 225009，China；² School of Horticulture and Plant Protection，Yangzhou University，Yangzhou，Jiangsu 225009，China；³Key Laboratory of Plant Functional Genomics，Ministry of Education，Yangzhou University，Jiangsu Yangzhou 225009，China

Received:2011-09-27 Revised:2011-12-27 Online:2012-02-25 Published:2012-02-25
Contact: CHEN Peng E-mail:chenpeng@yzu.edu.cn

Abstract

Abstract: Ginkgo biloba leaves were collected from Ginkgo Germplasm Resources Garden of Yangzhou University during different developmental periods，and were used to extract total RNA. After degenerate primer PCR and 5′ RACE amplifying，a 876 bp cDNA sequence containing 5′ end and start codon，named GbFOMT-13 was obtained，which had been embodied in GenBank database，and accession No. is JN711433. Alignment with BLAST of the nucleotide and deduced amino acid sequence and phylogenetic tree analysis showed that GbFOMT-13 was partial of FOMT gene from Ginkgo biloba. Semi-quantitative RT-PCR analysis revealed that GbFOMT-13 was developmental stage-specific and its expression level was the highest in the spring and the lowest in the summer，which is similar with the change of total flavonoid increase rate in Ginkgo biloba leaf.

Key words: Ginkgo biloba, leaf, GbFOMT-13, gene, cloning, expression pattern

CLC Number:

S 664.3

ZHANG Chuan-Li, CHEN Peng, ZHONG Yue-Ming, ZHOU Chang-Yuan, LIANG Guo-Hua, SHEN Dan-Hong, WU Qiu-Yue. Cloning and Expression of Flavonoid O-methyltransferase Gene in Ginkgo biloba[J]. ACTA HORTICULTURAE SINICA, 2012, 39(2): 355-362.

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Cloning and Expression of Flavonoid O-methyltransferase Gene in Ginkgo biloba

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