Development of Functional SNP Markers Anchored PpKO Gene in Pear

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (10): 1876-1884.

• Fruit Trees • Previous Articles Next Articles

Development of Functional SNP Markers Anchored PpKO Gene in Pear

TIAN Yi-Ke, WANG Cai-Hong, BAI Mu-Dan, YIN Hao, LI Jie-Fa

（College of Landscape and Horticulture，Qingdao Agricultural University，Qingdao，Shandong 266109，China）

Online:2012-10-25 Published:2012-10-25

Abstract

Abstract: The functional markers based on gene sequences are ideal markers for genotyping，gene mapping and marker assistant selection for associated traits. In this research，5 PCR primer pairs were designed according to the cDNA sequence of PpKO gene in pear which codes the protein of ent-kaurene oxidase（KO），one of the key enzymes in the path of gibberellin synthesis. These primer pairs were tested in the population derived from the cross of‘Aishengli’×‘Chili’by high resolution melting（HRM）ananlysis，and one of them was selected for genotyping. Furthermore，the fine genotyping function of this primer pair was confirmed by 9 cultivars. Sequencing and comparison of amplicons from the progenies and the 9 cultivars showed that the primer pair could amplified a fragment of 200 bp in length，which located in the eighth exon of PpKO gene，with 2 non-synonymous SNPs（single nucleotide polymor- phisms）detected. The two SNPs are genetic markers suitable for high resolution analysis，which are useful tools for genetic mapping，gene location and germplasm identification.

Key words: pear, single nucleotide polymorphism, functional marker, ent-kaurene oxidase gene

CLC Number:

S 661.2

TIAN Yi-Ke, WANG Cai-Hong, BAI Mu-Dan, YIN Hao, LI Jie-Fa. Development of Functional SNP Markers Anchored PpKO Gene in Pear[J]. ACTA HORTICULTURAE SINICA, 2012, 39(10): 1876-1884.

[1]	SONG Jiankun, YANG Yingjie, LI Dingli, MA Chunhui, WANG Caihong, and WANG Ran. A New Pear Cultivar‘Luxiu’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 3-4.
[2]	DONG Xingguang, CAO Yufen, ZHANG Ying, TIAN Luming, HUO Hongliang, QI Dan, XU Jiayu, LIU Chao, and WANG Lidong. A New Cold-resistant Crispy Pear Cultivar‘Yucuixiang’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 5-6.
[3]	OU Chunqing, JIANG Shuling, WANG Fei, MA Li, ZHANG Yanjie, and LIU Zhenjie. A New Early-ripening Pear Cultivar‘Xingli Mishui’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 7-8.
[4]	ZHANG Yanjie, WANG Fei, OU Chunqing, MA Li, JIANG Shuling, and LIU Zhenjie. A New Pear Cultivar‘Zhongli Yucui 3’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 9-10.
[5]	WANG Suke, LI Xiugen, YANG Jian, WANG Long, SU Yanli, ZHANG Xiangzhan, and XUE Huabai. A New Red Pear Cultivar‘Danxiahong’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 13-14.
[6]	WANG Fei, OU Chunqing, ZHANG Yanjie, MA Li, and JIANG Shuling. A New Late Ripening Pear Cultivar‘Huaqiu’with Long Storage Period [J]. Acta Horticulturae Sinica, 2022, 49(S1): 9-10.
[7]	SONG Jiankun, LI Dingli, YANG Yingjie, MA Chunhui, WANG Caihong, and WANG Ran. A New Pear Cultivar‘Qindaohong’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 11-12.
[8]	GUO Weizhen, ZHAO Jingxian, LI Ying. A New Mid-early Ripening Pear Cultivar‘Meiyu’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 2051-2052.
[9]	LIU Jinming, GUO Caihua, YUAN Xing, KANG Chao, QUAN Shaowen, NIU Jianxin. Genome-wide Identification of Dof Family Genes and Expression Analysis Sepal Persistent and Abscission in Pear [J]. Acta Horticulturae Sinica, 2022, 49(8): 1637-1649.
[10]	TAO Xin, ZHU Rongxiang, GONG Xin, WU Lei, ZHANG Shaoling, ZHAO Jianrong, ZHANG Huping. Fructokinase Gene PpyFRK5 Plays an Important Role in Sucrose Accumulation of Pear Fruit [J]. Acta Horticulturae Sinica, 2022, 49(7): 1429-1440.
[11]	LIANG Qin, ZHANG Yanhui, KANG Kaiquan, LIU Jinhang, LI Liang, FENG Yu, WANG Chao, YANG Chao, LI Yongyu. Molecular Evolution of MiR168 Family and Their Expression Profiling During Dormancy of Pyrus pyrifolia [J]. Acta Horticulturae Sinica, 2022, 49(5): 958-972.
[12]	XIANG Miaolian, WU Fan, LI Shucheng, MA Qiaoli, WANG Yinbao, XIAO Liuhua, CHEN Jinyin, CHEN Ming. Exogenous Melatonin Regulates Reactive Oxygen Metabolism to Induce Resistance of Postharvest Pear Fruit to Black Spot [J]. Acta Horticulturae Sinica, 2022, 49(5): 1102-1110.
[13]	SU Yanli, GAO Xiaoming, YANG Jian, WANG Long, WANG Suke, ZHANG Xiangzhan, XUE Huabai. Dynamic Changes of Browning Degree，Phenolics Contents and Related Enzyme Activities During Pear Fruit Development [J]. Acta Horticulturae Sinica, 2022, 49(11): 2304-2312.
[14]	YANG Bo, WEI Jia, LI Kunfeng, WANG Chengliang, NI Junbei, TENG Yuanwen, and BAI Songling. PpyERF060-PpyABF3-PpyMADS71 Regulates Ethylene Signaling Pathway- Mediated Pear Bud Dormancy Process [J]. Acta Horticulturae Sinica, 2022, 49(10): 2249-2262.
[15]	WANG Yaru, LI Yong, WANG Jin, WANG Yingtao, LI Xiao, and WANG Yongbo. A New Mid-ripening Pear Cultivar‘Jijin’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2771-2772.

Development of Functional SNP Markers Anchored PpKO Gene in Pear

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments