SSR Mining in Pepper（Capsicum annuum L.）Transcriptome and the Polymorphism Analysis

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (1): 168-174.

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SSR Mining in Pepper（Capsicum annuum L.）Transcriptome and the Polymorphism Analysis

LIU Feng1,3，WANG Yun-sheng2,3，TIAN Xue-liang3,4，MAO Zhen-chuan3，ZOU Xue-xiao1,*，and XIE Bing-yan3,*

（1Institute of Vegetables Hunan Academy of Agricultural Sciences，Changsha 410125，China；2College of Bio-Safety Science and Technology，Hunan Agricultural University，Changsha 410128，China；3Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081，China；4College of Henan Institute of Science and Technology，Xinxiang，Henan 453003，China）

Received:2011-11-11 Revised:2011-12-21 Online:2012-01-25 Published:2012-01-25
Contact: ZOU Xue-xiao，XIE Bing-yan

Abstract

Abstract: In this study，we investigated 221 037 unigenes（97.3 Mb）from two transcriptome in pepper using SSR finding soft. In total，17 319（7.83%）SSRs were identified from these SSR-containing unique ESTs with the occurring frequency of 1/56 kb. Among，single nucleotide type（56.30%）is the most abundant，followed by di-（19.16%），tri-（23.18%）. A total of 10 468 primer pairs were successfully designed and 1 538 primer pairs exhibited polymorphism by E-PCR 19 out of 20 primer pairs selected at random yielded amplification products，of which 7 primer pairs showed polymorphism among 10 different pepper varieties，PIC ranged from 0.33–0.89，average 0.67，and 10 pepper breeds were grouped into three classes. This results indicate that molecular markers will be more usable in map structure，analysis of genetic polymorphism in pepper.

Key words: pepper（Capsicum annuum L.）, transcriptome, EST-SSR

CLC Number:

S 641.3

LIU Feng;WANG Yun-sheng;TIAN Xue-liang;MAO Zhen-chuan;ZOU Xue-xiao;and XIE Bing-yan;. SSR Mining in Pepper（Capsicum annuum L.）Transcriptome and the Polymorphism Analysis[J]. ACTA HORTICULTURAE SINICA, 2012, 39(1): 168-174.

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SSR Mining in Pepper（Capsicum annuum L.）Transcriptome and the Polymorphism Analysis

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