Cloning and Expression Analysis of MADS box Gene in Sweet Cherry（Prunus avium）

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (8): 1462-1468.

• Fruit Trees • Previous Articles Next Articles

Cloning and Expression Analysis of MADS box Gene in Sweet Cherry（Prunus avium）

LIN Miao-miao，ZHAO Chang-zhu，JIANG Jian-fu，GU Hong，CHEN Jin-yong，and FANG Jin-bao*

Key Laboratory for Fruit Tree Growth，Development and Quality Control，Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，Zhengzhou 450009，China

Received:2011-05-17 Revised:2011-07-19 Online:2011-08-25 Published:2011-08-25
Contact: FANG Jin-bao*

Abstract

Abstract: A MADS box gene was isolated from the floral bud of sweet cherry（Prunus avium L.‘Lapins’）by using RT-PCR and RACE approaches，the gene was named PaMADS3（The accession number in GenBank：HQ229605）. The full length cDNA of PaMADS3 is 1 095 bp，with an ORF of 723 bp encoding 240 putative amino acid residues. Sequence analysis indicated that PaMADS3 shared highly homology with Arabidopsis SEP gene. Tissue specific analysis showed that PaMADS3 expressed in petal，stamen，and carpel. Real-time RT-PCR revealed that when the shoots were treated with 15 ℃ and 25 ℃，respectively，the expression of PaMADS3 was increased in carpel under the temperature of 25 ℃.　　expression

Key words: sweet cherry, flower development, MADS box, cloning

CLC Number:

S 662.5

LIN Miao-miao;ZHAO Chang-zhu;JIANG Jian-fu;GU Hong;CHEN Jin-yong;and FANG Jin-bao. Cloning and Expression Analysis of MADS box Gene in Sweet Cherry（Prunus avium）[J]. ACTA HORTICULTURAE SINICA, 2011, 38(8): 1462-1468.

[1]	TIAN Mingkang, XU Zhixiang, LIU Xiuqun, SUI Shunzhao, LI Mingyang, and LI Zhineng, . Identification of the AP2 Subfamily Transcription Factors in Chimonanthus praecox and the Functional Study of CpAP2-L11 [J]. Acta Horticulturae Sinica, 2023, 50(2): 382-396.
[2]	ZHANG Xiaoming, YAN Guohua, ZHOU Yu, WANG Jing, DUAN Xuwei, WU Chuanbao, and ZHANG Kaichun. A New Sweet Cherry Rootstock Cultivar‘Jingchun 2’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 31-32.
[3]	WANG Sha, ZHANG Xinhui, ZHAO Yujie, LI Bianbian, ZHAO Xueqing, SHEN Yu, DONG Jianmei, YUAN Zhaohe. Cloning and Functional Analysis of PgMYB111 Related to Anthocyanin Synthesis in Pomegranate [J]. Acta Horticulturae Sinica, 2022, 49(9): 1883-1894.
[4]	QIU Ziwen, LIU Linmin, LIN Yongsheng, LIN Xiaojie, LI Yongyu, WU Shaohua, YANG Chao. Cloning and Functional Analysis of the MbEGS Gene from Melaleuca bracteata [J]. Acta Horticulturae Sinica, 2022, 49(8): 1747-1760.
[5]	ZHENG Lin, WANG Shuai, LIU Yunuo, DU Meixia, PENG Aihong, HE Yongrui, CHEN Shanchun, ZOU Xiuping. Gene Cloning and Expression Analysis of NAC Gene in Citrus in Response to Huanglongbing [J]. Acta Horticulturae Sinica, 2022, 49(7): 1441-1457.
[6]	LIU Yaoyao, WU Yanyan, Shi Yan, MAO Tianyu, BAO Manzhu, ZHANG Junwei, ZHANG Jie. Preliminary Study on the Relationship Between Promoter Sequence Difference of PmTAC1 and Weeping Trait of Prunus mume [J]. Acta Horticulturae Sinica, 2022, 49(6): 1327-1338.
[7]	LI Lixian, WANG Shuo, CHEN Ying, WU Yingtao, WANG Yaqian, FANG Yue, CHEN Xuesen, TIAN Changping, FENG Shouqian. PavMYB10.1 Promotes Anthocyanin Accumulation by Positively Regulating PavRiant in Sweet Cherry [J]. Acta Horticulturae Sinica, 2022, 49(5): 1023-1030.
[8]	SONG Mengfei, ZHA Gaohui, CHEN Jinfeng, LOU Qunfeng. Research Progress on Molecular Basis of Plant Architecture Related Traits in Cucumber [J]. Acta Horticulturae Sinica, 2022, 49(12): 2683-2702.
[9]	LI Maofu, YANG Yuan, WANG Hua, FAN Youwei, SUN Pei, JIN Wanmei. Identification and Analysis of Self Incompatibility S-RNase in Rose [J]. Acta Horticulturae Sinica, 2022, 49(1): 157-165.
[10]	ZHANG Xiaoming, YAN Guohua, ZHOU Yu, WANG Jing, DUAN Xuwei, and ZHANG Kaichun, . A New Sweet Cherry Rootstock Cultivar‘Jingchun 1’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2787-2788.
[11]	ZHANG Xiaoming, YAN Guohua, ZHOU Yu, WANG Jing, DUAN Xuwei, and ZHANG Kaichun, . A New Sweet Cherry Rootstock Cultivar‘Landing 3’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2789-2790.
[12]	BIAN Shicun, LU Yani, XU Wujun, CHEN Boqing, WANG Guanglong, XIONG Aisheng. Garlic Circadian Clock Genes AsRVE1 and AsRVE2 and Their Expression Analysis Under Osmotic Stress [J]. Acta Horticulturae Sinica, 2021, 48(9): 1706-1716.
[13]	QI Xiliang, LIU Congli, SONG Lulu, LI Ming. Functional Analysis of Sucrose-phosphate Synthase Genes（SPS）in Sweet Cherry [J]. Acta Horticulturae Sinica, 2021, 48(8): 1446-1456.
[14]	MA Junjie, SONG Lina, LI Le, MA Xiaochun, JIN Lei, XU Weirong. VaCBL6 from Vitis amurensis Involved in Abiotic Stress Response and ABA-mediated Pathway [J]. Acta Horticulturae Sinica, 2021, 48(6): 1079-1093.
[15]	YU Hailong, REN Wenjing, FANG Zhiyuan, YANG Limei, ZHUANG Mu, LÜ Honghao, WANG Yong, JI Jialei, ZHANG Yangyong. Advances on Fertility Restoration for Cytoplasmic Male Sterility in Vegetables [J]. Acta Horticulturae Sinica, 2021, 48(5): 1031-1046.

Cloning and Expression Analysis of MADS box Gene in Sweet Cherry（Prunus avium）

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments