Analysis of Genetic Diversity Among Germplasm of Prunus mume Sieb. et Zucc. Using SRAP Markers

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (1): 117-124.

• Ornamental Plants • Previous Articles Next Articles

Analysis of Genetic Diversity Among Germplasm of Prunus mume Sieb. et Zucc. Using SRAP Markers

ZHANG Jun-wei1，MAO Qing-shan2，and BAO Man-zhu1,*

（1Key Laboratory of Horticultural Plant Biology，Ministry of Education，College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China；2Mei Flower Research Center of China，Wuhan 430074，China）

Received:2010-07-26 Revised:2010-12-20 Online:2011-01-25 Published:2011-01-25
Contact: BAO Man-zhu

Abstract

Abstract: In order to evaluate the genetic relationship among germplasm of Prunus mume Sieb. et Zucc.，sequence related amplified polymorphism（SRAP）marker was used to analyze genetic diversity of 135 samples of Mei. Seventeen primer pairs generated 124 bands，106 bands（87.5%）of which were polymorphic ones. SRAP primer pairs amplified 4 to 12 bands with an average of 7.3 bands per primer pair. Revealed by NTSYS software，the SM coefficient of genetic similarity ranged from 0.556 to 0.958，with an average of 0.733，which indicated that genetic basis was relatively abundance among the 135 Mei resources. A dendrogram was constructed based on SRAP data using UPGMA cluster method. The 135 samples of Mei were classified into 7 major groups，which was basically corresponded with the genetic relationships based on morphological traits.

Key words: SRAP marker, Prunus mume Sieb. et Zucc., genetic diversity

CLC Number:

S 685.17

ZHANG Jun-wei;MAO Qing-shan;and BAO Man-zhu;. Analysis of Genetic Diversity Among Germplasm of Prunus mume Sieb. et Zucc. Using SRAP Markers[J]. ACTA HORTICULTURAE SINICA, 2011, 38(1): 117-124.

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Analysis of Genetic Diversity Among Germplasm of Prunus mume Sieb. et Zucc. Using SRAP Markers

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