Molecular Cloning APX from Lilium longiforum and Overexpressing to Arabidopsis thaliana Enhanced Salt Tolerance

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (12): 1983-1983–1990.

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Molecular Cloning APX from Lilium longiforum and Overexpressing to Arabidopsis thaliana Enhanced Salt Tolerance

CHEN Li1，XIN Hai-bo1，SUN Xiang-rong2，YIN Hui1，LI Xiao-xin1，and YI Ming-fang1,*

（1Department of Ornamental Horticulture and Landscape Architecture，China Agricultural University，Beijing 100193，China；2Shaanxi Environmental Monitoring Center，Xi’an 710054，China）

Received:2010-03-26 Revised:2010-11-16 Online:2010-12-25 Published:2010-12-25
Contact: YI Ming-fang

Abstract

Abstract: An all-length cDNA of LlAPX gene was cloned from Lilium longiforum‘White Heaven’. The gene consisted of 1 074 bp encoding protein of 251 amino acids. Phylogenetic tree analysis showed that the APX had a highly conservative during evolution. We constructed a plant overexpression vector with LlAPX，and transgeniced to Arabidopsis thaliana. The APX activity in transgenic LlAPX Arabidopsis thaliana increased 4.7–7.8 fold，the AsA content increased 1.5–2.3 fold，and enhanced the salt tolerance in transgenic LlAPX Arabidopsis thaliana seeds.

Key words: Lilium longiforum, ascorbate peroxidase（APX）, cloning, salt tolerance

CLC Number:

S 682.2

CHEN Li;XIN Hai-bo;SUN Xiang-rong;YIN Hui;LI Xiao-xin;and YI Ming-fang;. Molecular Cloning APX from Lilium longiforum and Overexpressing to Arabidopsis thaliana Enhanced Salt Tolerance[J]. ACTA HORTICULTURAE SINICA, 2010, 37(12): 1983-1983–1990.

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Molecular Cloning APX from Lilium longiforum and Overexpressing to Arabidopsis thaliana Enhanced Salt Tolerance

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