Abstract: Some factors including plant growth regulator concentrations and combinations，illumination conditions and different additives were studied on Callistephus chinensis‘Bouquet Scarlet’leaves for plantlet regeneration. The results showed that：（1）Under light conditions，the appropriate medium for adventitious bud induction from leaves of C. chinensis‘Bouquet Scarlet’was MS + 3.0 mg · L-1 6-BA + 1.0 mg · L-1 IBA，the induction frequency was 56.7% and the adventitious bud number per explants was 3.3.（2）Although the adventitious buds from leaves didn’t benefit differentiate in darkness conditions，it was accelerate the formation of the leaf callus.（3）Some substances in the medium with proline，CH and NH4NO3 could promote the adventitious bud induction from calli. The suitable medium was MS + 1.0 mg · L-1 6-BA + 0.1 mg · L-1 IBA + 300 mg · L-1 proline，the induction frequency was 82.8% and the adventitious bud number per explants was 4.5.（4）On rooting induction medium（1/2MS + 0.10 mg · L-1 IBA），the rooting rates was 89.1% and the survial rates of regenerated plants was 89.3%.

Key words: Callistephus chinensis, leaf, callus, plant regeneration