Function Exploration of PpIDD11 in Regulating Peach Flower Development

doi:10.16420/j.issn.0513-353x.2021-0566

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1841-1852.doi: 10.16420/j.issn.0513-353x.2021-0566

• Research Papers • Next Articles

Function Exploration of PpIDD11 in Regulating Peach Flower Development

JIANG Yajun¹^,², CHEN Jiajia¹, TAN Bin¹^,³, ZHENG Xianbo¹^,³, WANG Wei¹^,³, ZHANG Langlang¹^,³, CHENG Jun¹^,³^,^*(), FENG Jiancan¹^,³^,^*()

¹College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China
²College of Forestry,Henan Agricultural University,Zhengzhou 450002,China
³Henan Key Laboratory of Fruit and Cucurbit Biology,Zhengzhou 450002,China

Received:2022-03-03 Revised:2022-05-13 Online:2022-09-25 Published:2022-10-08
Contact: CHENG Jun,FENG Jiancan E-mail:jcheng2007@163.com;jcfeng@henau.edu.cn

Abstract

Abstract:

In this study,an IDD transcription factor gene PpIDD11 was cloned from peach‘Qiumihong’. The coding sequence of PpIDD11 is 1 575 bp,it encodes 524 amino acids and has a conserved ID domain. The assay of subcellular localization showed that PpIDD11 was localized in the nucleus. PpIDD11 showed the transcriptional activation activity in yeast. Quantitative real-time PCR analysis indicated that PpIDD11 was mainly expressed in floral organ,with a highest transcript level in pistil. Overexpress PpIDD11 Arabidopsis exhibited a phenotype of higher stigma,shorter siliqua and fewer seeds compared with wild type. In addition,the transgenic lines showed a curled rosette leaves and dwarf phenotype. Transcriptome sequencing analysis showed that the PpIDD11 transgenic Arabidopsis line had 3 378 up-regulated genes and 3 156 down-regulated genes,including the regulatory genes（LOX4,LOX3,GLC,E6L1,etc）of floral organ development.

Key words: peach, PpIDD11, pistil, plant height, leaf curling

CLC Number:

S662.1

JIANG Yajun, CHEN Jiajia, TAN Bin, ZHENG Xianbo, WANG Wei, ZHANG Langlang, CHENG Jun, FENG Jiancan. Function Exploration of PpIDD11 in Regulating Peach Flower Development[J]. Acta Horticulturae Sinica, 2022, 49(9): 1841-1852.

Figures/Tables 10

References 40

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用途 Use	引物名称 Primer name	序列（5′-3′） Sequence
克隆 Clone	PpIDD11-F	TGGGGAAAGAAAACAAAGGAGA
克隆 Clone	PpIDD11-R	TCAATTAAACCTTTGCATTCTTAGG
亚细胞定位 Subcellular localization	PpIDD11-GFP-EcoRⅠ-F	GTGGATCCAAAGAATTCTCCTTTTTATCTGTATCTTCCTCCA
亚细胞定位 Subcellular localization	PpIDD11-GFP-EcoRⅠ-R	CTCCTTTACCCATGAATTCATTAAACCTTTGCATTCTTAGG
自激活鉴定 Self-activation	PpIDD11-BD-EcoRⅠ-F	CCGGAATTCTTTCCTTTTTATCTGTATCTTCCTCCA
自激活鉴定 Self-activation	PpIDD11-BD-PstⅠ-R	GCACTGCAGTCAATTAAACCTTTGCATTCTTAGG
过表达 Primers for overexpression sites	PpIDD11-SAK-EcoRⅠ-F	CCGGAATTCTGGGGAAAGAAAACAAAGGAGA
过表达 Primers for overexpression sites	PpIDD11-SAK-XhoⅠ-R	CCGCTCGAGTCAATTAAACCTTTGCATTCTTAGG
定量 qRT-PCR	PpIDD11-qPCR-F	ATGTATGGCGGGCTTTTATTC
定量 qRT-PCR	PpIDD11-qPCR-R	TGCTGATGCTGGTCATGTTG
定量桃内参 Primers for peach internal control	PpTEF2-qPCR-F	GGTGTGACGATGAAGAGTGATG
定量桃内参 Primers for peach internal control	PpTEF2-qPCR-R	TGAAGGAGAGGGAAGGTGAAAG
定量拟南芥内参 Primers for Arabidopsis internal control	AtUBC-qPCR-F	CTGCGACTCAGGGAATCTTCTAA
定量拟南芥内参 Primers for Arabidopsis internal control	AtUBC-qPCR-R	TTGTGCCATTGAATTGAACCC

用途 Use	引物名称 Primer name	序列（5′-3′） Sequence
克隆 Clone	PpIDD11-F	TGGGGAAAGAAAACAAAGGAGA
克隆 Clone	PpIDD11-R	TCAATTAAACCTTTGCATTCTTAGG
亚细胞定位 Subcellular localization	PpIDD11-GFP-EcoRⅠ-F	GTGGATCCAAAGAATTCTCCTTTTTATCTGTATCTTCCTCCA
亚细胞定位 Subcellular localization	PpIDD11-GFP-EcoRⅠ-R	CTCCTTTACCCATGAATTCATTAAACCTTTGCATTCTTAGG
自激活鉴定 Self-activation	PpIDD11-BD-EcoRⅠ-F	CCGGAATTCTTTCCTTTTTATCTGTATCTTCCTCCA
自激活鉴定 Self-activation	PpIDD11-BD-PstⅠ-R	GCACTGCAGTCAATTAAACCTTTGCATTCTTAGG
过表达 Primers for overexpression sites	PpIDD11-SAK-EcoRⅠ-F	CCGGAATTCTGGGGAAAGAAAACAAAGGAGA
过表达 Primers for overexpression sites	PpIDD11-SAK-XhoⅠ-R	CCGCTCGAGTCAATTAAACCTTTGCATTCTTAGG
定量 qRT-PCR	PpIDD11-qPCR-F	ATGTATGGCGGGCTTTTATTC
定量 qRT-PCR	PpIDD11-qPCR-R	TGCTGATGCTGGTCATGTTG
定量桃内参 Primers for peach internal control	PpTEF2-qPCR-F	GGTGTGACGATGAAGAGTGATG
定量桃内参 Primers for peach internal control	PpTEF2-qPCR-R	TGAAGGAGAGGGAAGGTGAAAG
定量拟南芥内参 Primers for Arabidopsis internal control	AtUBC-qPCR-F	CTGCGACTCAGGGAATCTTCTAA
定量拟南芥内参 Primers for Arabidopsis internal control	AtUBC-qPCR-R	TTGTGCCATTGAATTGAACCC

基因ID Gene ID	表达差异 log₂（IDD11/WT）	名称 Name	功能 Function	参考文献 Reference
AT1G21910	2.77242	DREB26	植物生长发育 Plant growth and development	Krishnaswamy et al.,2011
AT3G18010	2.40747	WOX1	分生组织发育 Meristematic tissue development	Zhang et al.,2011
AT1G65450	-3.77981	GLC	参与植物受精过程 Involved in the process of plant fertilisation	Leshem et al.,2012
AT3G12500	-3.07184	ATHCHIB	叶片形态发育Leaf blade morphology development	Robles et al.,2001
AT2G33850	-2.78991	E6L1	参与植物早期授粉过程 Involved in the early pollination process of plants	Doucet et al.,2019
AT1G72520	-2.61478	LOX4	花器官发育 Floral organ development	Caldelari et al.,2011
AT1G17420	-2.54068	LOX3	花器官发育 Floral organ development	Caldelari et al.,2011

基因ID Gene ID	表达差异 log₂（IDD11/WT）	名称 Name	功能 Function	参考文献 Reference
AT1G21910	2.77242	DREB26	植物生长发育 Plant growth and development	Krishnaswamy et al.,2011
AT3G18010	2.40747	WOX1	分生组织发育 Meristematic tissue development	Zhang et al.,2011
AT1G65450	-3.77981	GLC	参与植物受精过程 Involved in the process of plant fertilisation	Leshem et al.,2012
AT3G12500	-3.07184	ATHCHIB	叶片形态发育Leaf blade morphology development	Robles et al.,2001
AT2G33850	-2.78991	E6L1	参与植物早期授粉过程 Involved in the early pollination process of plants	Doucet et al.,2019
AT1G72520	-2.61478	LOX4	花器官发育 Floral organ development	Caldelari et al.,2011
AT1G17420	-2.54068	LOX3	花器官发育 Floral organ development	Caldelari et al.,2011

Function Exploration of PpIDD11 in Regulating Peach Flower Development

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