Abstract: Style S-determinants have been determined to be S-RNase and the SFB ( S-haplotype-specific F-box gene) alleles are considered to be the first candidates of the pollen S-determinants. A total length SFB gene (GenBank accession No1FJ362525) named AcSFBd was cloned with RT-PCR and RACE technolgy from the anther of almond Yingzui p lanted in Xinjiang. AcSFBd was 1 125 bp in length encoding a protein of 374 amino acids. This gene encoded proteins with the F-box motif in the N amino terminus, and with four ( hyper) variable regions, i e. , V1, V2, Hva and Hvb which were similarwith S-RNase genes, considered to be recognition site between SFB and S-RNase. With the bioinformatics analysis, the AcSFBd protein was 43 kD,was instable, hydrophilicity and non-secretory protein and had ligase enzyme activity. After transformed to Escherichia coli BL21 and induced by isopropyl-β-D-thiogalactopyranoside ( IPTG) , recombinant protein about
60 kD was expressed in pET-AcSFB system and separated by SDS-PAGE electrophoresis

Key words: almond, gene, clone, SFB gene, p rokaryotic exp ression, bioinformatics