Polyploid Induction in Pear in vitro Treatment With Gamma-rays

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (2): 257-260.

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Polyploid Induction in Pear in vitro Treatment With Gamma-rays

SUN Qing-rong^1*,SUN Hong-yan¹,ZHU En-yuan², and LI Lin-guang¹

(¹Shandong Institute of Pomology, the State Agriculture Ministry Experiment Station of Temperate Fruits Germplasm Key Outdoor Observation in Taian, Shandong Fruits Biotechnology Breeding Key Lab, Tai’an 27100,China; ²Department of Science and Technology of Shandong Province, Jinan250014,China)

Received:2008-08-06 Revised:2008-12-10 Online:2009-02-25 Published:2009-02-25
Contact: SUN Qing-rong

Abstract

Abstract: In vitro shoots of pear cultivar ‘Fertility’(Pyrus communis L.) were irradiated with gamma rays. Three different explants of shoot tip, lateral bud and young leaf from irradiated shoots were cultured separately. Shoots from 3 different explants were subcultured and selected. Morphological mutants with thicker stem, shorter internode, smaller leaf index and shorter petiole were obtained.Chromosome counting confirmed that the mutants were triploids and tetraploids. The lengths of stomatal guard cells and specific leaf mass of polyploids were significantly increased than that of diploids.

Key words: pear, in vitro shoots, gamma-rays, polyploid

CLC Number:

S 661.2

SUN Qing-rong;SUN Hong-yan;ZHU En-yuan;and LI Lin-guang. Polyploid Induction in Pear in vitro Treatment With Gamma-rays [J]. ACTA HORTICULTURAE SINICA, 2009, 36(2): 257-260.

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Polyploid Induction in Pear in vitro Treatment With Gamma-rays

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