Abstract: Interspecific relationship s of 88 cultivars and 1 wild species of Osmanthus fragrans were evaluated by using sequence-related amplified polymorphism ( SRAP ) marker, with O. heterophyllus (G. Don. ) P. S. Green and O. cooperi Hemsl. as contrast species. Eighteen primer pairs produced 296 loci, out of which 248 were polymorphic, the percentage of polymorphic loci was 83.78%. An average of 16.4 loci and 13.8 polymorphic lociwere generated by each pair of primers. The Shannonps index (0.3412) and genetic diversity (0.2191) within Albus Group were the highest among 4 groups of O. fragrans. A high
level of genetic differentiation among O. fragrans groups was detected based on Nei's genetic diversity analysis (52.95% ). The cluster analysis using arithmetic averages (UPGMA ) showed that six clades existed in O. fragrans groups when genetic similarity coefficient was given as 0.762. The genetic distance between Asiaticus Group and other 3 groups were far. Cultivars with deep color in Luteus Group often clustered together with several cultivars in Aurantiacus Group. Cluster analysis based on SRAP marker is generally consistent with taxonomic result based on morphology.

Key words: Osmanthus fragrans, SRAP-PCR, genetic diversity, cluster analysis