Detection of Apple chlorotic leaf spot virus in Pear by Primed in situ Labeling

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (1): 15-20.

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Detection of Apple chlorotic leaf spot virus in Pear by Primed in situ Labeling

ZHAO Ying and NIU Jian-xin *

(Agricultural College, Shihezi University, Shihezi,Xinjiang 832003 ,China)

Received:2008-06-05 Revised:2008-11-17 Online:2009-01-25 Published:2009-01-25
Contact: NIU Jian-xin

Abstract

Abstract: The slices of pear leaf samples infected by apple chlorotic leaf spot virus were prepared for situ RT-PCR and were detected by primed in situ labeling technique using special primer. It was showed that both staining methods of PRINS-FITC and PRINS-Rhodamine can get good detection results in which the parts have viruses showed green and red fluorescence light, respectively, and the sites of showing fluorescence showed the same sites as the sites detected by RT-PCR method. Therefore, primed in situ labeling technique can be perfectly used for virus in situ detection of fruit trees, and it is also a rapid, simple and reliable in situ detection method.

Key words: pear, primed in situ labeling, Apple chlorotic leaf spot virus, virus detection method, staining

CLC Number:

S 661.2

ZHAO Ying and NIU Jian-xin . Detection of Apple chlorotic leaf spot virus in Pear by Primed in situ Labeling[J]. ACTA HORTICULTURAE SINICA, 2009, 36(1): 15-20.

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Detection of Apple chlorotic leaf spot virus in Pear by Primed in situ Labeling

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