Cloning of the Terminal Regions of Apple stem pitting virus in Pear Trees Using 3' and 5' RACE

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (4): 493-500.

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Cloning of the Terminal Regions of Apple stem pitting virus in Pear Trees Using 3' and 5' RACE

ZHAO Ying and NIU Jian-xin*

(Department of Horticulture, Agricultural College of Shihezi University, Shihezi, Xinjiang 832003, China)

Received:2007-08-20 Revised:2008-02-27 Online:2008-04-25 Published:2008-04-25
Contact: NIU Jian-xin

Abstract

Abstract: The 5' and 3' terminal regions of Apple stem pitting virus (ASPV) in tree of Pyrus sinkiangensis ‘Kuerle’pear were cloned using rapid amplification of cDNA ends (RACE) technique. The results showed that the techniques of 3' and 5' RACE could amplify the terminal regions of ASPV in pear trees effectively, and the 329 bp and 367 bp segments of ASPV were obtained. The 3' terminal region of ASPV in Kuerle pear contained 131 nt of non-coding sequence and a poly (A) tail, and the 5' terminal region contained 34 nt of non-coding sequence. They shared 78% and 84% nucleotide acid identity with the sequence of NC_003462 in NCBI nucleotide acid database, respectively. This study laid a sound foundation for obtaining the full sequence and analyzing the function and structure of genome of ASPV in pear trees.

Key words: pear, Apple stem pitting virus, rapid amplification of 3' and 5' terminal regions, poly (A) tail

CLC Number:

S 661.2

ZHAO Ying and NIU Jian-xin. Cloning of the Terminal Regions of Apple stem pitting virus in Pear Trees Using 3' and 5' RACE[J]. ACTA HORTICULTURAE SINICA, 2008, 35(4): 493-500.

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Cloning of the Terminal Regions of Apple stem pitting virus in Pear Trees Using 3' and 5' RACE

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