Development of Fingerprinting Key and Analysis of Genetic Diversity with SSR Marker for Sweet Cherry Cultivars

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (3): 329-336.

Development of Fingerprinting Key and Analysis of Genetic Diversity with SSR Marker for Sweet Cherry Cultivars

ZHANG Qi-jing^{1, 2} , ZHANG Xin-zhong³ , DA IHong-yan¹ , GU Da-jun² , YAN Gui-jun⁴ , L I Chun-min³ , and ZHANG Zhi-hong^1*

(¹College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; ²Liaoning Institute of Pomology, Xiongyue, Liaoning 115214, China; ³Changli Fruit Research Institute, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China; ⁴ The University of Western Australia, Perth 6009, Australia)

Received:2007-09-01 Revised:2008-01-12 Online:2008-03-25 Published:2008-03-25
Contact: ZHANG Zhi-hong

Abstract

Abstract:

SSR technique was used to develop fingerprinting key and study the genetic diversity on sweet cherry. Eighteen pairs of primers cloned from cherry, peach and apricot amplified a total of 83 alleles among the 19 cultivars of sweet cherry ( Prunus avium ) and 2 cultivars of ground cherry ( P. fruticosa). The number of alleles amplified per primer pair ranged from 2 to 8, with a mean of 416. The polymorphic information content values ( PIC) ranged from 0.38 to 0.80, with a mean of 0.64. Seven sweet cherry cultivars showed unique bands or banding patterns. A fingerp rinting key developed by 4 pairs of p rimers: UDP98-414, UDP98-406, UDP96-001 and PMS40 could discriminate 18 sweet cherry cultivars. UPGMA cluster analysis of genetic distance separated the 19 cultivars of sweet cherry into two group s. The phylogeny of sweet cherry cultivars revealed the closed relationships among cultivars tested.

Key words: sweet cherry, SSR, genetic diversity, cultivar identification, fingerprinting key

CLC Number:

S 662.5

ZHANG Qi-jing;;ZHANG Xin-zhong;DA IHong-yan;GU Da-jun;YAN Gui-jun;L I Chun-min;and ZHANG Zhi-hong. Development of Fingerprinting Key and Analysis of Genetic Diversity with SSR Marker for Sweet Cherry Cultivars[J]. ACTA HORTICULTURAE SINICA, 2008, 35(3): 329-336.

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Development of Fingerprinting Key and Analysis of Genetic Diversity with SSR Marker for Sweet Cherry Cultivars

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