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ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (6): 1485-1490.

• 观赏植物 • Previous Articles     Next Articles

Overexpression of a Novel Member of ERF TranscriptionFactor JERF3 in Lily Enhances the Tolerance to Salt

YANG Yu-hong1,YIN Li-rong3,GE Hong1,HUANG Rong-feng2,XIAO Qi-ming3, and XIE Bing-yan1*   

  1. (1 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081,China; 2Institute of Biotechnology Research, Chinese Academy of Agricultural Sciences, Beijing 100081,China; 3 College of Bio-sefty Science and Technology, Hunan Agricultural University, Hunan Changsha 410128,China)
  • Received:2007-04-26 Revised:2007-06-24 Online:2007-12-25 Published:2007-12-25

Abstract:

Using the callus of lilium scales, we established an optimum agrobacterium-mediated transformation system after comparative assays among agrobacterium concentration, cultivation time and antibiotics sensitivity. With this system, an ERF family novel member JERF3 was transferred into lily. Identification using PCR amplification, RT-PCR and Southern blot assay sindicated that the JERF3 gene was integrated into lily's genome and transcriptionally expressed. And overexpression of JERF3 in lily effectively enhances the tolerance to salt.

Key words: Lily, Transcription factor, JERF3 gene, Tolerance to salt

CLC Number: