Identification of S-genotype of Ten Cultivars from Niitaka Line Pear

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (6): 1353-1360.

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Identification of S-genotype of Ten Cultivars from Niitaka Line Pear

YUAN De-yi¹,TAN Xiao-feng^1*,ZHANG Lin¹,ZHAO Si-dong¹,WUYUN Ta-na¹, DUAN Jing-hua¹,and CAO Yu-fen²

(¹The Key Laboratory of Non-wood Forest Product of Forestry Ministry, Central-South of Forestry and Technology University, Changsha 410004, China;² Fruit Tree Research Institute，Chinese Academy of Agricultural Sciences，Xingcheng 125100，China)

Received:2007-05-07 Revised:2007-09-30 Online:2007-12-25 Published:2007-12-25

Abstract

Abstract: Ten cultivars with extremely close relationship from Niitaka line pear were used as materials for S-genotyping. In addition, two cultivars 'Imamuraaki' and 'Amanogawa' that were considered to be the parents of cultivar 'Niitaka' were also included. PCR-RFLP analysis of genomic DNAs of the 12 cultivars, nucleotide sequencing of amplified fragments and sequence analysis were performed. As the results, the ten cultivars of Niitaka line were genotyped as follows: 'Niitaka' (S₃S₉), 'WhangKeumBae' (S₃S₄), 'Sujeong' (S₃S₉), 'Josengwhangkeum'(S₄S₉), 'Soumituniitaka'(S₃S₉), 'Yewang'(S₃S₉), 'Jinqiu'(S₃S₉), 'Banndainiitaka' (S₃S₉), 'Nokaniitaka'(S₃S₉), and 'Sunhwang' (S₃S₅). The S-genotype of 'Amanogawa' and 'Imamuraaki'was identified as S₁S₉and S₁S₆, respectively, suggesting that 'Imamuraaki'is unlikely to be the pollen parent of 'Niitaka'.

Key words: Pear, Pyrus pyrifolia, Niitaka line pear, Self-incompatibility, S-genotype, Identification

CLC Number:

S 661.2

YUAN De-yi;TAN Xiao-feng;ZHANG Lin;ZHAO Si-dong;WUYUN Ta-na;DUAN Jing-hua;and CAO Yu-fen. Identification of S-genotype of Ten Cultivars from Niitaka Line Pear[J]. ACTA HORTICULTURAE SINICA, 2007, 34(6): 1353-1360.

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Identification of S-genotype of Ten Cultivars from Niitaka Line Pear

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