Efficient Somatic Embryogenesis from Leaf Explants of Phalaenopsis in vitro Culture and Histological Observations

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 431-436.

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Efficient Somatic Embryogenesis from Leaf Explants of Phalaenopsis in vitro Culture and Histological Observations

CUI Guang-rong^1,2,HOU Xi-lin^1*,ZHANG Zi-xue²,ZHANG Cong-yu²,HU Neng-bing²,and LIU Yue-cheng²

(¹College of Horticulture Nanjing Agricultural University,Nanjing 210095，China;²Plant Science Department of Anhui Science and Technology University, Fengyang,Anhui 233100,China )

Received:2006-08-23 Revised:2007-03-12 Online:2007-04-25 Published:2007-04-25
Contact: HOU Xi-lin

Abstract

Abstract: The leaves of phalaenopsis were used as explants to culture in the 1/2MS basic medium with different kinds of plant growth regulators for somatic embryos induction. The effects of different concentrations of three kinds of plant growth regulators 6-BA, Ad, NAA and their combinations on somatic embryogenesis were studied in vitro culture. Meanwhile, the influences of the concentrations of sucrose (w/v) and coconut water (v/v) on somatic embryogenesis were studied too. The results showed that the effects of cytokinins N6-benzyl adenine (6-BA) was better than adenine sulphate (Ad) in embryoid induction. The auxin а-naphthaleneacetic acid (NAA) combined with given concentrations of 6-BA and Ad could effectively raise the frequency of somatic embryogenesis. The optimum plant growth regulator combination for somatic embryogenesis was 6-BA 4.0 mg/L +Ad 2.0-4.0 mg/L+NAA 1.0-2.0mg/L. 20-40 g/L sucrose (w/v) and 200 ml/L coconut water (v/v) added in the medium was benefit for somatic embryogenesis. The results of histological observations indicated that somatic embryos originated from upper epidermis and mesophyll single cell nearby stoma. The course of somatic embryos development was similar to other plants. Finally, the somatic embryos became protocorm-like body.

Key words: Phalaenopsis, Leaf, Somatic embryo, Plant tissue culture, Histological observation, Protocorm-like body

CLC Number:

S 682.31

CUI Guang-rong;HOU Xi-lin;ZHANG Zi-xue;ZHANG Cong-yu;HU Neng-bing;and LIU Yue-cheng. Efficient Somatic Embryogenesis from Leaf Explants of Phalaenopsis in vitro Culture and Histological Observations[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 431-436.

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Efficient Somatic Embryogenesis from Leaf Explants of Phalaenopsis in vitro Culture and Histological Observations

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