Clon ing and Sequence Analysis of Two Encoding Ethylene Receptor cDNAs in ‘Dongzao’Jujube

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 333-338.

• 果树 • Previous Articles Next Articles

Clon ing and Sequence Analysis of Two Encoding Ethylene Receptor cDNAs in ‘Dongzao’Jujube

WEI Shao-chong, PENG Fu-tian, SHU Huai-rui^*, and SUN Xu-dong

( Key Laboratory of Crop Biology of Shandong Province, College of Horticulture Science and Engineering, Shandong Agricultural University, Taipan, Shandong 271018, China)

Received:2006-06-12 Revised:2006-12-28 Online:2007-04-25 Published:2007-04-25
Contact: SHU Huai-rui

Abstract

Abstract: Two cDNA fragments with 1 058 bp were amp lified from Chinese jujube ( Z izyphus jujuba‘Dongzao’) fruit at a half2red mature stage through RT2PCR using degenerated p rimers, and then both 5′encoding region and 3′2 end with a UTR ( untranslated region) of these two cDNA fragment sequences were obtained. The obtained ethylene recep tor genes with two open reading frames, referred to ZjETR1 and ZjERS1,encoded 738 and 632 amino acids, respectively. Sequence analysis indicated that ZjETR1 and ZjERS1 shared
a high similarity with other ethylene recep tors at the polypep tide level, and could be divided into the ETR1 and ERS1 sub-groups.

Key words: Jujube, Ethylene recep tor, Fruit ripening, Sequence analysis, Gene cloning

CLC Number:

S 665.1

WEI Shao-chong;PENG Fu-tian;SHU Huai-rui;and SUN Xu-dong. Clon ing and Sequence Analysis of Two Encoding Ethylene Receptor cDNAs in ‘Dongzao’Jujube[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 333-338.

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Clon ing and Sequence Analysis of Two Encoding Ethylene Receptor cDNAs in ‘Dongzao’Jujube

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