Effects of Picloram, ABA and TDZ on Somatic Embryogenesis of Banana

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (1): 81-86.

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Effects of Picloram, ABA and TDZ on Somatic Embryogenesis of Banana

WEI Yue-rong^1,2; YANG Hu¹ ;HUANG Bing-zhi¹ ;HUANG Xia² ;HUANG Xue-lin² ;QIU Ji-shui¹ ;
XU Lin-bing¹

(¹ Fruit Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; ² The Key Laboratory ofGene Engineering of Ministry of Education, School of Life Sciences, Zhongshan University, Guangzhou 510275, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

Abstract

Abstract: Establishment of a stable embryogenic cell suspension ( ECS) is a prerequisite for biotechno
logical breeding of banana. Although many important p rogress has been made, but app lication of embryogenic
cell suspension for genetic imp rovement ofM usa is limited because of low induction percentage of embryogeno
ic callus, low conversion frequence of p lant regeneration from the somatic embryos. Therefore, an op timal pro
tocol for embryogenic cell suspensions is still required to establish. In this study the effects of Picloram, ABA
and TDZ on somatic embryogenesis of banana from youngmale flower of Musa acuminata cv. Mas (AA) were
investigated. Results showed that the induction percentage of embryogenic calli reached 15.6% when 2, 4-D
in the callus induction medium was substituted by 8.28 μmol/L picloram. However, the addition ofABA into
somatic embryo development medium inhibited somatic embryogenesis from embryogenic cell suspension and
resulted in callus formation from somatic embryos. Treatmentwith TDZ at concentration of 0.2 μmol/L resul
ted in increase of the percentage of germination of somatic embryos from 17.28% to 72.77% and the plant
conversion ratio from 16.49% to 67.05% , respectively.

Key words: Banana, Somatic embryogenesis, Picloram, ABA, TDZ

WEI Yue-rong;YANG Hu;HUANG Bing-zhi;HUANG Xia;HUANG Xue-lin;QIU Ji-shui;XU Lin-bing. Effects of Picloram, ABA and TDZ on Somatic Embryogenesis of Banana[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 81-86.

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Effects of Picloram, ABA and TDZ on Somatic Embryogenesis of Banana

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