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ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (6): 1341-1344.

• 研究报告 • Previous Articles     Next Articles

cDNA-AFLP Analysis on Genes Associated with Vernalization in Vitro inBrassica campestris ssp. chinensis

Sun Baojuan1, 2;Cao Jiashu1;Huang Xisong1;Yu Xiaolin1   

  1. (1 Institute of Vegetable Science, Zhejiang University, Hangzhou, Zhejiang 310029, China; 2Vegetable Research Inseitute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China)
  • Received:2006-01-12 Revised:2006-03-27 Online:2006-12-25 Published:2006-12-25
  • Contact: Cao Jiashu

Abstract: Fifteen ESTs associated with vernalization in vitro of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) were isolated by cDNA-AFLP. Blast results showed that 12 ESTs down-regulated by vernalization had high similarity to genes encoding enzymes involved in the disassimilation. 3 ESTs up regulated had high similarity to geneswith positive effect on vernalization, including ribosomal protein subunit related to the synthesis of priotein or the linkage factor of FAC involved in auto flowering pathway, the
subunit of ATPase, and the subuint of small-G-protein.

Key words: Chinese cabbage-pak-choi, Brassica campestris ssp. chinensis, Vernalization, In vitro, Gene, cDNA-AFLP

CLC Number: