Abstract: Studies were carried out on high-frequency shoot regeneration from leaves of Fuyu and Jiro,two Japanese non-astringent persimmon. The effects of growth regulator and cultural time on the adventitious shoot regeneration from leaves were investigated. The results showed that TDZ was a strong and efficient hormone inducing adventitious shoot and calli formation. The best regeneration was achieved in 1 /2DKW + TDZ 0.5 mg·L ^{- 1} + ZT 1.0 mg·L ^{- 1} , in which callus percentage was 96% and shooting was near above 80%.
After 30 day culture, the calliwith small shootingwere transferred to 1 /2DKW medium supp lemented with 0.5 mg·L ^{- 1} zeatin and 0.1 mg·L ^{- 1} IAA. Shooting regeneration rate were more than 96% , and average adventitious shoots per exp lantwere 7.8 and 5.4 respectively for Fuyu and Jiro after 30 days culture. Rooting percentage from regenerated shootswas about 75%.

Key words: Persimmon, TDZ, Leaf, Plant regeneration, Callus