Abstract: The eight CP genes of CMV isolated from different areas and vegetables in China were amplified by one-step RT-PCR, and transformed into pMD-18T vector and then sequenced, respectively. The results showed that the sequences of the eight CP genes were all 657 nucleotides that could encode the protein of 218 amino acids of each isolate, and the isolates shared high sequence homology from 92.8% to 99.1% among them. The nucleotide sequence alignment showed, compared with some CP gene sequences of CMV reported in GenBank, the eight CP genes shared 90.7% - 98.0% homology with those of CMV subgroup I strains, whereas only shared 75.8% - 78.1% homology with those of CMV subgroup Ⅱ strains. Therefore, it was supposed that all the eight CMV isolates reported in this study belonged to the subgroup I of CMV.

Key words: Cucumber mosaic virus, CP Gene, RT-PCR, Sequence analysis