Cryopreservation of in Vitro Cultured Kiwifruit Shoot-tips by Vitrification and their Regeneration

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 842-844.

• 研究报告 • Previous Articles Next Articles

Cryopreservation of in Vitro Cultured Kiwifruit Shoot-tips by Vitrification and their Regeneration

Xu Xiaobiao¹;Gu Qingqing¹;Cai Zuguo¹;Deng Xiaomei²;Zhang Qiuming³

(¹Agronomy College of Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; ²Institute of B iotechnology Research, Jiangxi Academy of Forestry Sciences, Nanchang, Jiangxi 330032, China; ³College of Horticulture and Gardening, Hunan Agricultural University, Changsha, Hunan 410128, China)

Received:2005-05-24 Revised:2005-09-19 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: Experiments were conducted to provide an appropriate means for long-term p reservation of kiwifruit germp lasm using cryopreservation by vitrification. Shoot-tips of a dwarf genotype of kiwifruit were cultured in vitro to obtain aseptic clones. The results showed that shoot-tips 1.5 - 2.5 mm in length with one or two leaf primordium were precultured in 5% DMSO + 5% sucrose + MS medium for 4 days. Pretreated shoottips with 2 mol/L glycerol + 0.4 mol/L sucrose for 30 min at room temperature, followed by dehydration with PVS2 (30% glycerol + 15% ethylene glycol + 15% DMSO + 0.4 mol/L sucrose) for 40 min at 0℃ and rapidly put into liquid nitrogen for 24 h. The defrosted shoot-tips were then inoculated onto subculture medium.The survival and regeneration rate of shoot-tips treated in thisway was 56.7% and 51.6% respectively. The
plantlets could normally root and survive after transplanting.

Key words: Kiwifruit, Shoot-tip, Vitrification, Cryopreservation

Xu Xiaobiao;Gu Qingqing;Cai Zuguo;Deng Xiaomei;Zhang Qiuming. Cryopreservation of in Vitro Cultured Kiwifruit Shoot-tips by Vitrification and their Regeneration[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 842-844.

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Cryopreservation of in Vitro Cultured Kiwifruit Shoot-tips by Vitrification and their Regeneration

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