Abstract: ‘Puyu’is a new yellow-fleshed kiwifruit cultivar selected from the crossbreeding of Actinidia chinensis‘Huayou’and Actinidia deliciosa‘K56’. The fruit shape is cylindrical with light brown fruit skin and short hairs. The fruit weight is of 106 g in average and can reach the maximum of 148 g. Its flesh is yellow，juicy，and aromatic with soluble solids content of 18%–20%，total sugar content of 11.5%，titratable acid content of 1.5% and vitamin C content of 1.724 mg ? g-1. The ripening period is in early October at the North part of Qinling Mountain and the fruit yield is about 30 t ? hm-2.

Key words: kiwifruit, yellow flesh, cultivar