In Vitro Culture and Plant Regeneration of Orychophragmus diffusus

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (5): 679-681.

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In Vitro Culture and Plant Regeneration of Orychophragmus diffusus

Wu Jun;Qi Xinghua;Wang Yi;Luo Qin;Wang Maohua;Yang Yi;Li Xufeng*; Tan Zhongming

( College of Life Science , Sichuan University , Chengdu 610064 , China)

Received:2003-11-24 Revised:2004-02-16 Online:2004-10-25 Published:2004-10-25

Abstract

Abstract: The different ways of regeneration of leaves and petioles in vitro were studied in Orychophragmus diffusus Z. M. Tan et J . M. Xu. On the MS medium with 6-BA and NAA , the leaves and petioles gave the high regeneration rate. When they were cultured on the MS medium with 2,4-D , 6-BA and NAA , they gave the high induction rate of the calli. The calli were differentiated easily on the medium with 6-BA. The embryonic plant s were reproduced rapidly in the same medium. Complete plant s were induced on the rooting medium. This study showed O. diffusus has apparent regeneration capacity.

Key words: Orychophragmus diffusus, Petiole, Leaf, Organogenesis, Plant regeneration

CLC Number:

S 63

Wu Jun;Qi Xinghua;Wang Yi;Luo Qin;Wang Maohua;Yang Yi;Li Xufeng;Tan Zhongming. In Vitro Culture and Plant Regeneration of Orychophragmus diffusus[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 679-681.

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In Vitro Culture and Plant Regeneration of Orychophragmus diffusus

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