Molecular Cloning and Sequence of Acid Invertase Gene Fragments(CMCW1) from Clivia miniata

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (2): 256-258.

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Molecular Cloning and Sequence of Acid Invertase Gene Fragments(CMCW1) from Clivia miniata

Wang Qiong;Wang Yan;Cheng Shuiyuan;Fei Yongjun;Jin Weibin

( Department of Horticulture,Yangzhou University，Yangzhou 225009，China；Department of Horticulture，Hubei Agricultural College，Jingzhou 434025，China；Key Laboratory of Waterlogged Disaster and Wetland Agriculture in Hubei Province，Jingzhou 434025，China)

Received:2003-04-24 Revised:2003-07-28 Online:2004-04-25 Published:2004-04-25

Abstract

Abstract: A pair of primers，designed from conserved regions of plant acid invertase genes，was used to amplify same gene by polymerase chain reaction(PCR)．As a result，one 518 bp fragment was obtained，and then cloned into pGEM-TEasy and sequenced．It has no intron and encoded 172 amino acids．This
gene has been registered in GenBank with accession number AY151269．The deduced amino acid sequence is higher identical to the gene from other plant．

Key words: Clivia miniata, Invertase, Gene, Cloning

CLC Number:

S 68

Wang Qiong;Wang Yan;Cheng Shuiyuan;Fei Yongjun;Jin Weibin. Molecular Cloning and Sequence of Acid Invertase Gene Fragments(CMCW1) from Clivia miniata[J]. ACTA HORTICULTURAE SINICA, 2004, 31(2): 256-258.

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Molecular Cloning and Sequence of Acid Invertase Gene Fragments(CMCW1) from Clivia miniata

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