RAPD Identification of Different Carnation Cultivars

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (1): 109-111.

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RAPD Identification of Different Carnation Cultivars

Su Youbo¹;Lin Chun²;Mao Jing¹;Mo XiJun³;Yang Ming³

(¹College of Resources and Environment , Yunnan Agricultural University , Kunming 650201 , China ; ² College of Agriculture and Biologic Technology , Yunnan Agricultural University , Kunming 650201 , China ; ³ Horticultural Institute , Yunnan Academy of Agricultural Sciences , Kunming 650201 , China)

Received:2003-05-09 Revised:2003-10-30 Online:2004-02-25 Published:2004-02-25

Abstract

Abstract: The technique of random amplified polymorphic DNA (RAPD) was used to amplify the genomic DNA fragments of 87 carnation cultivars by 4 selected arbitrary 10-mer primers , 2113 bands were obtained , and the size range was 500 - 4300 bp. The average number of DNA bands amplified by each primer was 915 cluster analysis showed that the extensive genetic variation existed among 87 cultivars of carnation. The 87 cultivars were divided into 10 groups at DNA level. This result has significance for the genetic breeding and identification of cultivars of carnation

Key words: Carnation ( Dianthus caryophyllus L. ), RAPD, Genetic diversity

CLC Number:

S 68

Su Youbo;Lin Chun;Mao Jing;Mo XiJun;Yang Ming. RAPD Identification of Different Carnation Cultivars[J]. ACTA HORTICULTURAE SINICA, 2004, 31(1): 109-111.

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RAPD Identification of Different Carnation Cultivars

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