Abstract: The factors which affect tip meristem culture , rapid propagation and the induction of in vitro stolon of manyflower Betony ( Stachys floridana Schuttl . ex Benth. ) were studied. The results showed that the optimum medium for tip meristem culture were MS + sucrose 4 % + 6-BA 0.5mg·L ^{- 1} + NAA 0.1 mg·L ^{- 1} with 74.0 %plant let ratio. MS supplemented with 6-BA 3.0 mg·L ^{- 1} and NAA 0.5 mg·L ^{- 1} was successful for propagation , each explant produced up to 5.6 seedlings. Total darkness , 20 ℃and MS medium with 6-BA 5.0 mg·L^{- 1} + CCC 500 mg·L ^{- 1} were better for the induction of in vitro stolon.

Key words: Manyflower Betony ( Stachys floridana Schuttl . ex Benth. ), Tip meristem culture, Propagation, Stolon induction