Identification of Molecular Genetic Markers Tightly Linked to White Rot Resistant Genes in Chinese Wild Grape

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (1): 6-10.

• 研究论文 • Previous Articles Next Articles

Identification of Molecular Genetic Markers Tightly Linked to White Rot Resistant Genes in Chinese Wild Grape

Xu Yan¹;Wang Yuejin¹;Zhou Peng²;Zhang Jianxia¹

(¹College of Horticulture , Northwest Sci-Tech university of Agriculture and Forestry , Yangling 712100 , China ;² National Key biotechnology laboratory for tropical cropes , Chinese Academy of Tropical Agricultural Sciences , Haikou 571101 , China)

Received:2002-03-26 Revised:2002-07-22 Online:2003-02-25 Published:2003-02-25

Abstract

Abstract: Bulk segregant analysis (BSA) , randomly amplified polymorphic DNA (RAPD) and sequnence characterized amplified region (SCAR) methords were used to tag the white rot-resistant genes of grape with 17 individuals of F₁progeny between Tangwei ( Vitis davidii ) , white rot-resistant and Ugni Blanc (V.vinifera) ,White rot-sensitive and 16 individuals self-pollinated of Tangwei . Among 155 random primers gave distinct band Patterns , One RAPD marker OPP09-760 was tightly linked to a major gene resistant to Coniothyrium diplodiella.Marker OPP09-760 was cloned and sequenced. According to the Sequence, two specific primers were designed to amplify all plant materials. RAPD marker was converted into SCAR marker. One distinct single band only in resistant plants was amplified and its size was the same as that of the RAPD marker. The SCAR marker’s popularity was confirmed. And it could be used for the identification of hybrid resistant to Coniothyrium diplodiella.

Key words: Grape, Chinese wild grape, White rot, RAPD, SCAR, Resistant genes

CLC Number:

Xu Yan;Wang Yuejin;Zhou Peng;Zhang Jianxia. Identification of Molecular Genetic Markers Tightly Linked to White Rot Resistant Genes in Chinese Wild Grape[J]. ACTA HORTICULTURAE SINICA, 2003, 30(1): 6-10.

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Identification of Molecular Genetic Markers Tightly Linked to White Rot Resistant Genes in Chinese Wild Grape

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