Leaf Culture and Plantlet Regeneration of Pear (Pyrus)

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (4): 367-368.

• 研究报告 • Previous Articles Next Articles

Leaf Culture and Plantlet Regeneration of Pear (Pyrus)

Xu Lingfei¹, Ma Fengwang¹, Wang Zhezhi², Ren Xiaolin¹, and Cao Xiaoyan²

(¹ College of Horticulture , Northwest Sci-Tec University of Agriculture and Forestry , Yangling 712100 , China ; ²College of life Science , Shaanxi Normal University , Xi’an 710062 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

Abstract

Abstract: Adventitious bud was regenerated from in vitro leaf culture of four pear scion cultivars. 20 days of dark culture was conducted , it was proved that using leaf of upper part of the plantlet as explant , and plating leaf
with abaxial layer downside may significantly enhanced the regeneration efficiency of leaf . The regeneration frequency and mean number of bud per leaf of‘Bayuehong’pear were 100 % and 5. 78 on NN69 + TDZ 1. 0 mg/ L + IBA 0. 5 mg/ L. The regenerated plants of‘Bayuehong’and‘Suisho’pear rooted in the 1/ 2 MS media supplemented with IBA.

Key words: Pear, Leaf culture, Plantlet regeneration

CLC Number:

S 661. 2

Xu Lingfei;Ma Fengwang;Wang Zhezhi;Ren Xiaolin;and Cao Xiaoyan. Leaf Culture and Plantlet Regeneration of Pear (Pyrus)[J]. ACTA HORTICULTURAE SINICA, 2002, 29(4): 367-368.

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Leaf Culture and Plantlet Regeneration of Pear (Pyrus)

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