Abstract: Eighty-nine tradit ional cultivars of Chinese chestnut were investigated in present study for genet icdiversity, cultivar identification and genet icrelationships. Fifteen isozyme loci of 9 enzymes were examined using isoelectric focusing in thin􀀁layer polyacrylamide gels. The parameters of genetic diversity and genetic distance were calculated by Biosys 1 and henogram was constructed by UPGMA clustering analysis with NTSYS software. A high genetic diversity was observed in major chestnut production provinces, such as Zhejiang, Shandong, Hubei and Jiangsu. Eighty􀀁four out of total 89 cultivars examined can be uniquely identified by 15 multi􀀁locus isozymes. The
UPGMA phenogram showed that the majority of cultivar of Shandong, Hubei, Jiangsu and Henan province was clustered together. The traditional cult ivars of Chinese chestnut were inclined to clustering of provincial groups.

Key words: Abstract: Key words: Chinese chestnut, Isozyme, Genetic diversity, Cultivar identification, Cluster analysis