Abstract: Raphnus sativus-antifungal proteins ( Rs-AFPs ) are cysteine􀀁rich, antifungal proteins. The Rs-AFP₁₍₂₎ genes were inserted into the vector pTrxFus and transferred into E. coli GI724. The fusion protein from recombiant pTrxAFP_{1( 2)} was about 22 kD and taking up 0. 4 and 0. 5 percent of the total proteins respectively. Inhibition experiment showed that the expressed products had antifungal activity to Colletotrichum musae. Expressed products from pTrxAFP₂ showed much stronger inhibitory activity than that from pTrxAFP1. A plant expression vector pBIAFP₂ was constructed, which had been transferred into tomato by Agrobactrium mediated transformation. 32 plantlets had been obtained and 28 of them detected by PCR and Southern Blot. The results showed that 17 of them were positive, and the results of Southern Blot showed 6 of them were positive.

Key words: Raphnus sativus-antifungal proteins ( Rs-AFPs), Gene expression, E. coli, Transgenic, Tomato