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Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (6): 1275-1289.doi: 10.16420/j.issn.0513-353x.2021-0306

• Research Papers • Previous Articles     Next Articles

Promoting Effects of Uniconazole on Grafting Formation of Tomato Seedlings and Underlying Mechanisms

MENG Xianmin1,2, CUI Qingqing1, DUAN Yundan1, ZHUANG Tuanjie1, PU Dan1, DONG Chunjuan1, YANG Wencai2, SHANG Qingmao1,*()   

  1. 1Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
    2Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops,Department of Vegetable Science,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China
  • Received:2021-11-30 Revised:2022-02-18 Online:2022-06-25 Published:2022-07-04
  • Contact: SHANG Qingmao E-mail:shangqingmao@caas.cn

Abstract:

Uniconazole(S3307),a gibberellin inhibitor,is often used to regulate plant growth and development,however,its effect on graft formation remains unclear. Here,tomato scion‘Yingfen 8’and stock‘Zhenai 1’were foliar sprayed twice with 0.25 mg · L-1 uniconazole on 15 d and 21 d after sowing. The results showed that uniconazole inhibited the growth of scion and stock seedlings,the plant height decreased by 20.58% and 12.75% respectively compared with the control with deionized water at 27 d after sowing,that is 0 h after grafting;from 12 h to 72 h after grafting,uniconazole reduced the contents of GA15 and GA8,promoted the accumulation of GA3,IAA,MEIAA,ICAld,TRP and iP7G;and it increased the expression difference in IAA1 above and below the graft union,GA20ox1,IAA1,and ARR17 expression was up-regulated above the graft union,while GA20ox1 and ARR17 expression were down-regulated below. From 6 h to 168 h after grafting,uniconazole also increased the relative expression of TMO6 and CyclinB1;2 both above and below the graft union,and WIND1,WOX4 and VND7 expression above;uniform expression of IAA1,ARR17,WIND1,TMO6,WOX4 and VND7 above and below the graft union occurred earlier,and xylem and phloem of the graft union were connected in advance. In addition,the graft union of uniconazole began to connect at 72 h after grafting,the connection between xylem and phloem was basically completed at 96-120 h after grafting,which was 24 h earlier than control. These results suggested that foliar sprayed uniconazole before grafting promoted tomato graft formation by regulating endogenous hormone levels in the graft union.

Key words: tomato, uniconazole, hormone, graft formation

CLC Number: