Screening and Isolation of Growth-Promoting Bacteria in Onion Rhizosphere and the Growth-Promoting Effect of Composite Microbial Agents on Onion

doi:10.16420/j.issn.0513-353x.2025-0684

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (9): 2733-2751.doi: 10.16420/j.issn.0513-353x.2025-0684

• Cultivation · Physiology & Biochemistry • Previous Articles Next Articles

Screening and Isolation of Growth-Promoting Bacteria in Onion Rhizosphere and the Growth-Promoting Effect of Composite Microbial Agents on Onion

YAO Wenxia¹, FAN Xiangjun¹, HAN Jia¹, LI Hui¹, LU Yang¹, YANG Hongyu¹^,^*(), ZHANG Yuxin²

¹ College of Horticulture， Gansu Agricultural University， Lanzhou 730070，Gansu， China
² Gansu Academy of Agricultural Sciences， Lanzhou 730070，Gansu， China

Received:2026-03-23 Revised:2026-05-20 Online:2026-09-25 Published:2026-09-20
Contact: YANG Hongyu

Abstract

Abstract:

In order to screen onion rhizosphere growth-promoting bacteria and explore their growth-promoting effects after being made into compound microbial agents，the onion rhizosphere soil in Hexi area was used as the material，and the growth-promoting bacteria were isolated and screened by functional medium. A total of 25 strains were isolated and screened，all of which had phosphorus- solubilizing ability，among which YC-342 had the strongest phosphorus-solubilizing ability（455.833 μg · mL^-1）. 15 strains had nitrogen fixation function，and the nitrogenase activity of JQ-MY-42 strain was the highest（90.02 nmol · h^-1 · mL^-1）. Seven strains had the ability to secrete IAA，among which JQ-MY-41 had the highest secretion（3.590 μg · mL^-1）. The strains were identified by 16S rDNA sequence. Six dominant strains were selected to prepare four kinds of compound microbial agents，and their growth-promoting effects were verified by pot experiment. After comprehensive evaluation，the TE formula（JQ-MY-41 + JQ-MY-42 + YC-342）showed the best performance among the four composite microbial agents：its phosphorus solubility reached 611.70 μg · mL^-1，which was significantly higher than other formulas. Compared with the control，the plant height，stem diameter，dry weight and fresh weight of aboveground and underground parts of onion were significantly increased. The activity of superoxide dismutase（SOD）in leaves reached 380.34 U · g^-1，which was significantly higher than that of the control. The activity of catalase（CAT）increased by 35%，the activity of ascorbate peroxidase（APX）increased by 38.54%，and the activity of peroxidase（POD）decreased by 48.83%. The content of MDA in leaves decreased to 0.81 μmol · g ^-1，which was significantly lower than that of the control（1.02 μmol · g^-1）. The contents of chlorophyll a，chlorophyll b and carotenoids increased by 25.71%，43.24% and 26.92%，respectively，and the total chlorophyll content reached 1.85 mg · g^-1，which was significantly higher than that of the control（1.41 mg · g^-1）. The Chl a/b ratio（2.50）and Car/Chl ratio（0.18）were not significantly different from the control. The activities of catalase（S-CAT），sucrase（S-UC），urease（S-UE）and alkaline phosphatase（S-ALP）in rhizosphere soil increased by 123.08%，148.19%，58.16% and 136.36%，respectively. Principal component analysis showed that TE treatment had the highest comprehensive score （0.7478），which was the optimal formula for onion rhizosphere growth-promoting bacteria compound microbial inoculants.

Key words: onion, rhizosphere growth-promoting bacteria, screening, compound microbial inoculant, hexi region, pot experiment, principal component analysis

CLC Number:

S633.2

YAO Wenxia, FAN Xiangjun, HAN Jia, LI Hui, LU Yang, YANG Hongyu, ZHANG Yuxin. Screening and Isolation of Growth-Promoting Bacteria in Onion Rhizosphere and the Growth-Promoting Effect of Composite Microbial Agents on Onion[J]. Acta Horticulturae Sinica, 2026, 53(9): 2733-2751.

Figures/Tables 14

References 53

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菌株 Strain	溶磷量/（μg · mL^-1） Phosphorus-solubilizing capacity	固氮酶活性/（nmol · h^-1 · mL^-1） Nitrogenase activity	IAA分泌量/（μg · mL^-1） IAA secretion amount	pH
JQ-MY-41	75.833	14.617	3.590	4.42
JQ-MY-1	48.782	2.642	0.729	5.69
JQ-MY-42	318.013	90.021	—	5.45
JQ-271	420.962	7.134	1.184	4.31
JQ-232(4)	76.090	—	—	4.82
JQ-142	77.628	—	—	5.40
JQ-232(2)	5.449	—	—	5.37
JQ-6412	238.397	—	—	4.72
JQ-232	225.064	49.488	0.238	6.24
JQ-MY-2	56.474	85.649	—	5.34
GT-5522	21.218	—	—	5.78
GT-142	52.628	—	—	5.49
GT-5521	18.782	—	—	5.81
GT-331	263.782	—	—	4.44
GT-441	455.577	7.134	—	4.32
GT-151	161.859	24.782	—	5.04
LJ-51	21.090	20.037	0.039	5.80
LJ-41	126.346	—	—	5.05
YC-4101	111.218	2.734	2.734	5.19
YC-142	78.526	3.909	—	5.34
YC-4211	454.167	12.152	2.567	4.11
YC-142(2)	449.551	—	—	4.23
YC-342	455.833	4.824	—	5.64
YC-161	14.295	5.355	—	6.27
YC-561	13.782	5.110	—	6.25

菌株 Strain	溶磷量/（μg · mL^-1） Phosphorus-solubilizing capacity	固氮酶活性/（nmol · h^-1 · mL^-1） Nitrogenase activity	IAA分泌量/（μg · mL^-1） IAA secretion amount	pH
JQ-MY-41	75.833	14.617	3.590	4.42
JQ-MY-1	48.782	2.642	0.729	5.69
JQ-MY-42	318.013	90.021	—	5.45
JQ-271	420.962	7.134	1.184	4.31
JQ-232(4)	76.090	—	—	4.82
JQ-142	77.628	—	—	5.40
JQ-232(2)	5.449	—	—	5.37
JQ-6412	238.397	—	—	4.72
JQ-232	225.064	49.488	0.238	6.24
JQ-MY-2	56.474	85.649	—	5.34
GT-5522	21.218	—	—	5.78
GT-142	52.628	—	—	5.49
GT-5521	18.782	—	—	5.81
GT-331	263.782	—	—	4.44
GT-441	455.577	7.134	—	4.32
GT-151	161.859	24.782	—	5.04
LJ-51	21.090	20.037	0.039	5.80
LJ-41	126.346	—	—	5.05
YC-4101	111.218	2.734	2.734	5.19
YC-142	78.526	3.909	—	5.34
YC-4211	454.167	12.152	2.567	4.11
YC-142(2)	449.551	—	—	4.23
YC-342	455.833	4.824	—	5.64
YC-161	14.295	5.355	—	6.27
YC-561	13.782	5.110	—	6.25

菌株编号 Strain Number	病原菌菌落直径/cm Pathogenic Bacterium Colony Diameter	抑菌率/% Bacteriostatic rate
CK	5.0 ± 0.1 a	—
JQ-MY-41	4.5 ± 0.2 ab	10.24 ± 0.30 e
JQ-MY-42	4.4 ± 0.1 ab	12.16 ± 0.24 d
JQ-271	4.3 ± 0.1 b	14.10 ± 0.20 c
JQ-232	4.6 ± 0.1 a	8.16 ± 0.26 f
YC-342	4.1 ± 0.1 c	18.04 ± 0.16 a
GT-151	4.2 ± 0.1 bc	16.06 ± 0.22 b

菌株编号 Strain Number	病原菌菌落直径/cm Pathogenic Bacterium Colony Diameter	抑菌率/% Bacteriostatic rate
CK	5.0 ± 0.1 a	—
JQ-MY-41	4.5 ± 0.2 ab	10.24 ± 0.30 e
JQ-MY-42	4.4 ± 0.1 ab	12.16 ± 0.24 d
JQ-271	4.3 ± 0.1 b	14.10 ± 0.20 c
JQ-232	4.6 ± 0.1 a	8.16 ± 0.26 f
YC-342	4.1 ± 0.1 c	18.04 ± 0.16 a
GT-151	4.2 ± 0.1 bc	16.06 ± 0.22 b

指标 Index	载荷 Load
指标 Index	PC1	PC2
地上鲜质量 AFM	0.089	0.092
地上干质量 ADM	0.087	0.071
地下鲜质量 UFM	0.083	0.163
地下干质量 UDM	0.079	0.186
根系活力 RA	-0.056	0.072
株高 PH	0.087	-0.054
茎粗 SD	0.089	-0.076
超氧化物歧化酶 SOD	0.093	-0.012
过氧化物酶 POD	-0.052	0.258
过氧化氢酶 CAT	0.042	0.296
抗坏血酸过氧化物酶 APX	0.033	-0.323
土壤蔗糖酶 S-UC	0.093	-0.043
土壤脲酶 S-UE	0.091	0.007
土壤碱性磷酸酶 S-ALP	0.089	-0.057
土壤过氧化氢酶 S-CAT	0.088	-0.065
特征值 Eiges value	10.649	2.806
方差贡献率/% Proportion of variance	70.993	18.704
累积方差贡献率/% Cumulative variance	70.993	89.697

Screening and Isolation of Growth-Promoting Bacteria in Onion Rhizosphere and the Growth-Promoting Effect of Composite Microbial Agents on Onion

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处理 Treatment	PC1（F₁）	PC2（F₂）	综合得分（F） Composite score	得分排名 Ranking
CK	-1.26081	0.00682	-0.893811	5
TB	0.75686	1.09501	0.742128	2
TC	-0.38553	0.86549	-0.111818	3
TE	1.24888	-0.74213	0.747809	1
TF	-0.3594	-1.22518	-0.484306	4

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