Effects of Spermidine on PSⅡ Function and Expression of Key Genes of Chlorophyll Metabolism in Common Bean Seedlings Under Low Temperature Stress

doi:10.16420/j.issn.0513-353x.2025-0102

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (3): 697-708.doi: 10.16420/j.issn.0513-353x.2025-0102

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Effects of Spermidine on PSⅡ Function and Expression of Key Genes of Chlorophyll Metabolism in Common Bean Seedlings Under Low Temperature Stress

CAO Jinping, YANG Wenhuan, LIU Xiujuan, WANG Yuping^*()

College of Horticulture，Gansu Agricultural University，Lanzhou 730070，China

Received:2025-07-29 Revised:2025-12-22 Online:2026-03-25 Published:2026-03-20
Contact: WANG Yuping

Abstract

Abstract:

In this study，the effects of 1.0 mmol · L^-1 exogenous spermidine（Spd）and methylglyoxal diamidine hydrazone（MGBG）on the growth，chlorophyll content，photosystemⅡ（PSⅡ）function，chlorophyll degradation and synthase activity and related gene expression of common bean seedlings under low temperature stress were investigated at 12 ℃/8 ℃. The results indicated that compared with the control，low-temperature stress inhibited the growth of seedlings；chlorophyll content maximum photochemical efficiency of PSⅡ（F_v/F_m），photochemical quenching coefficient（q_P），electron transport rate（ETR）and actual photochemical efficiency（Φ_PSII）were all significantly reduced（P < 0.05）；while non-photochemical quenching coefficient（q_N）significantly increased（P < 0.05）. Under low-temperature stress，the chlorophyll fluorescence induction（OJIP）curve showed significant alterations：the fluorescence intensities at the J- and P-steps were reduced，while the heat dissipation per unit cross-section（DIo/CSm）and energy dissipation per reaction center（DIo/RC）were markedly elevated. Under low temperatures（T2），the application of Spd reversed the aforementioned trends，with significant increases in light energy absorption（ABS/CSm），conversion（TRo/CSm），and electron transport（ETo/CSm）. Spd-induced downregulation of key chlorophyll degradation genes PvCLH1（chlorophyllase 1）/PvCLH2 （chlorophyllase 2）and PvRCCR1（red chlorophyll metabolite reductase 1）resulted in decreased chlorophyllase（CLH）activity；Key chlorophyll synthesis genes PvPOR1（proto-deplastidic chlorophyll reductase 1）/PvPOR2（proto-deplastidic chlorophyll reductase 2）showed significantly increased relative expression（P < 0.05），with elevated chlorophyll acidase activity. The results indicate that exogenous Spd alleviates the suppression of photosynthesis in common bean seedlings under low-temperature stress by upregulating the expression of chlorophyll synthesis-related genes，increasing chlorophyll content，and enhancing the absorption and utilization of light energy by PSⅡ.

Key words: common bean, low temperature stress, chlorophyll metabolism, spermidine, PSⅡ, chlorophyll fluorescence kinetics curve（OJIP）

CAO Jinping, YANG Wenhuan, LIU Xiujuan, WANG Yuping. Effects of Spermidine on PSⅡ Function and Expression of Key Genes of Chlorophyll Metabolism in Common Bean Seedlings Under Low Temperature Stress[J]. Acta Horticulturae Sinica, 2026, 53(3): 697-708.

Figures/Tables 9

References 15

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基因 Gene	正向引物序列（5′-3′） Forward Primer	反向引物序列（5′-3′） Reverse Primer
Actin	GAAGTTCTCTTCCAACCATCC	TTTCCTTGCTCATTCTGTCCG
PvCLH1	CCACTCTGACATGCTAGATGATGATAC	TGACTCCTCCAACAAACCTCCTC
PvCLH2	ATTGTCATTGCTCCTCAGTTGTACA	AGACTCACATGCATGTCTCTAATGG
PvRCCR1	ATGATCCGCAGTAGTCCAACAATG	GAGTGTCCAATTCAGTGTCCTCATAG
PvPOR1	TCTCCAGCAGTCACCAAGTCTAC	CTCAGCCAAAGCCTTAGCAGTG
PvPOR2	GGTCTTTCATTGTCAGAACCTAGCA	GTCCTAGATGGTTTGTTCCAACACT

基因 Gene	正向引物序列（5′-3′） Forward Primer	反向引物序列（5′-3′） Reverse Primer
Actin	GAAGTTCTCTTCCAACCATCC	TTTCCTTGCTCATTCTGTCCG
PvCLH1	CCACTCTGACATGCTAGATGATGATAC	TGACTCCTCCAACAAACCTCCTC
PvCLH2	ATTGTCATTGCTCCTCAGTTGTACA	AGACTCACATGCATGTCTCTAATGG
PvRCCR1	ATGATCCGCAGTAGTCCAACAATG	GAGTGTCCAATTCAGTGTCCTCATAG
PvPOR1	TCTCCAGCAGTCACCAAGTCTAC	CTCAGCCAAAGCCTTAGCAGTG
PvPOR2	GGTCTTTCATTGTCAGAACCTAGCA	GTCCTAGATGGTTTGTTCCAACACT

处理 Treatment	叶绿素a/（mg· g^-1 FW） Chlorophyll a	叶绿素b/（mg · g^-1FW） Chlorophyll b	叶绿素（a + b）/（mg · g^-1FW） Chlorophyll（a + b）	叶绿素a/b Chlorophyll a/b
对照 Control	2.02 ± 0.05 b	0.61 ± 0.03 b	2.63 ± 0.04 b	1.75 ± 0.04 e
T1	2.66 ± 0.06 a	0.71 ± 0.04 a	3.37 ± 0.05 a	1.22 ± 0.03 f
T2	0.67 ± 0.03 e	0.38 ± 0.02 d	1.05 ± 0.02 e	3.29 ± 0.07 b
T3	1.60 ± 0.04 c	0.53 ± 0.03 c	2.13 ± 0.03 c	2.73 ± 0.05 d
T4	0.35 ± 0.02 f	0.29 ± 0.01 e	0.64 ± 0.02 f	3.72 ± 0.09 a
T5	1.10 ± 0.03 d	0.40 ± 0.02 d	1.51 ± 0.03 d	3.05 ± 0.06 c

处理 Treatment	叶绿素a/（mg· g^-1 FW） Chlorophyll a	叶绿素b/（mg · g^-1FW） Chlorophyll b	叶绿素（a + b）/（mg · g^-1FW） Chlorophyll（a + b）	叶绿素a/b Chlorophyll a/b
对照 Control	2.02 ± 0.05 b	0.61 ± 0.03 b	2.63 ± 0.04 b	1.75 ± 0.04 e
T1	2.66 ± 0.06 a	0.71 ± 0.04 a	3.37 ± 0.05 a	1.22 ± 0.03 f
T2	0.67 ± 0.03 e	0.38 ± 0.02 d	1.05 ± 0.02 e	3.29 ± 0.07 b
T3	1.60 ± 0.04 c	0.53 ± 0.03 c	2.13 ± 0.03 c	2.73 ± 0.05 d
T4	0.35 ± 0.02 f	0.29 ± 0.01 e	0.64 ± 0.02 f	3.72 ± 0.09 a
T5	1.10 ± 0.03 d	0.40 ± 0.02 d	1.51 ± 0.03 d	3.05 ± 0.06 c

处理 Treatment	天线吸收的光能用于电子传递的效率 ψEo	I点相对可变荧光强度 V_i	J点相对可变荧光强度 V_j	Q_A还原速率 dV/dto	以吸收光能为基础的性能指数 PI_ABS
对照 Control	0.406 ± 0.018 a	0.490 ± 0.006 d	0.443 ± 0.007 d	1.060 ± 0.025 f	1.467 ± 0.026 a
T1	0.379 ± 0.008 b	0.553 ± 0.022 c	0.467 ± 0.023 cd	1.140 ± 0.015 e	1.363 ± 0.015 b
T2	0.302 ± 0.006 d	0.883 ± 0.012 a	0.533 ± 0.015 b	1.357 ± 0.012 b	0.410 ± 0.015 d
T3	0.348 ± 0.003 c	0.680 ± 0.020 b	0.493 ± 0.009 bc	1.217 ± 0.012 d	1.330 ± 0.021 b
T4	0.242 ± 0.006 e	0.907 ± 0.003 a	0.643 ± 0.022 a	1.513 ± 0.018 a	0.313 ± 0.019 e
T5	0.309 ± 0.002 d	0.727 ± 0.038 b	0.523 ± 0.009 b	1.277 ± 0.018 c	1.273 ± 0.009 c

Effects of Spermidine on PSⅡ Function and Expression of Key Genes of Chlorophyll Metabolism in Common Bean Seedlings Under Low Temperature Stress

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处理 Treatment	ABS/CSm	DIo/CSm	ETo/CSm	TRo/CSm	ABS/RC	DIo/RC	ETo/RC	TRo/RC
对照Control	3 094.33 ± 39.84 a	306.67 ± 3.19 e	796.33 ± 11.72 a	1 422.33 ± 15.86 a	2.92 ± 0.05 a	0.25 ± 0.02 d	1.90 ± 0.05 a	2.48 ± 0.01 a
T1	2 891.00 ± 37.82 b	342.67 ± 4.33 d	768.67 ± 11.35 ab	1 350.00 ± 15.48 b	2.63 ± 0.04 b	0.29 ± 0.02 cd	1.73 ± 0.06 b	2.40 ± 0.01 b
T2	1 718.33 ± 23.29 d	439.33 ± 8.35 b	410.00 ± 6.12 d	968.00 ± 10.92 e	2.55 ± 0.03 b	0.36 ± 0.02 b	1.11 ± 0.03 d	2.30 ± 0.02 c
T3	1 845.67 ± 25.55 c	358.00 ± 4.89 d	715.33 ± 11.2 b	1 241.67 ± 13.43 c	2.43 ± 0.03 c	0.34 ± 0.02 bc	1.31 ± 0.11 c	2.26 ± 0.02 c
T4	1 582.00 ± 20.11 e	478.00 ± 9.29 a	342.67 ± 5.36 e	934.33 ± 10.01 e	2.45 ± 0.02 c	0.43 ± 0.01 a	1.05 ± 0.02 d	2.27 ± 0.01 c
T5	1 759.67 ± 23.79 cd	385.67 ± 7.76 c	518.33 ± 8.89 c	1 117.00 ± 11.39 d	2.22 ± 0.02 d	0.34 ± 0.03 bc	1.19 ± 0.01 cd	2.19 ± 0.02 d

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