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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (10): 1859-1872.doi: 10.16420/j.issn.0513-353x.2021-0476

• Research Papers • Previous Articles     Next Articles

A Novel R2R3-MYB Transcription Factor Gene AaMYB6 Involved in Anthocyanin Biosynthesis in Anthurium andraeanum

LI Chonghui1,3, YANG Guangsui1,3, ZHANG Zhiqun1,3, YIN Junmei2,3,*()   

  1. 1Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture,Haikou 571101,China
    2Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China
    3The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization,Danzhou,Hainan 571737,China
  • Received:2021-07-19 Revised:2021-09-02 Online:2021-10-25 Published:2021-11-01
  • Contact: YIN Junmei E-mail:yinjunmei2004@163.com

Abstract:

A new R2R3-MYB gene AaMYB6 with GenBank accession number MZ171064 was isolated from the spadix of Anthurium andraeanum‘Pink Champion’based on the information from previous transcriptome data for identification of transcription factor genes associated with anthocyanin biosynthesis. The function of AaMYB6 was analyzed through the assays such as amino acid sequence alignment,phylogenetic tree analysis,subcellular localization,overexpression in tobacco,qRT-PCR,yeast two-hybrid assay(Y2H),and bimolecular fluorescence complementation(BiFC). The results suggested that AaMYB6 showed homology with previously identified AaMYB1 from anthurium and belong to C1 subgroup R2R3-MYBs,represented by Zea may C1,that regulate anthocyanin biosynthesis in monocots. AaMYB6 was proved to be nucleus-localized protein. Overexpression of AaMYB6 in tobacco markedly activated the expression of the enzyme genes NtF3′H,NtDFR,NtANS,NtUFGT,specifically involved in anthocyanin biosynthesis in filaments and promoted the accumulation of anthocyanin pigments. AaMYB6 was mainly expressed in the spadix of‘Rapido’. And the expression of AaMYB6 was closely linked to anthocyanin accumulation in the spadix of various cultivars. The interaction between AaMYB6 and the AabHLH1 protein,which was identified previously to regulate the biosynthesis of anthocyanin and proanthocyanidin in anthurium,was confirmed by Y2H and BiFC assays. The results indicated that AaMYB6 play a role in the regulation of anthocyanin biosynthesis in anthurium.

Key words: Anthurium andraeanum, MYB transcription factor, anthocyanin, heterologous overexpression, transcriptional regulation, gene function

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