Genome-Wide Identification and Expression Analyses of PP2C Gene Family in Dendrobium catenatum

doi:10.16420/j.issn.0513-353x.2021-0382

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (12): 2458-2470.doi: 10.16420/j.issn.0513-353x.2021-0382

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Genome-Wide Identification and Expression Analyses of PP2C Gene Family in Dendrobium catenatum

ZHANG Tingting¹, LI Yuxin¹, ZHANG Deyao¹, KANG Yuqian¹, WANG Jian², SONG Xiqiang², ZHOU Yang¹^,^*()

¹Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops,School of Horticulture,Hainan University,Haikou 570228,China
²Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants/Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province,School of Forestry,Hainan University,Haikou 570228,China

Received:2021-07-20 Revised:2021-08-26 Published:2022-01-04
Contact: ZHOU Yang E-mail:zhouyang@hainanu.edu.cn

Abstract

Abstract:

The protein phosphatase 2C（PP2C）family genes of Dendrobium catenatum Lindl. were identified and analyzed by bioinformatics method. The expression patterns of PP2C genes in different tissues and under various abiotic stresses and hormones were analyzed by quantitative real-time PCR （qRT-PCR）. A total of 67 family members were identified,which encode 80 to 1 075 amino acids,and the molecular weight were between 8.86 to 119.59 kD,and the isoelectric points ranged from 4.13 to 9.36. The PP2C proteins were divided into 13 subgroups by phylogenetic tree analysis,and the conserved motifs of each subgroup were similar. The number of exons in PP2C gene ranged from 1 to 15. Spatial expression pattern analysis showed that four PP2Cs had the highest expression in gynostemium of D. catenatum,and five genes had the highest expression in capsule and one gene had the highest expression in root,indicating that the expression of PP2C was tissue-specific. The expression levels of DcPP2C5,DcPP2C20 and DcPP2C56 were significantly up-regulated by drought stress（20% PEG6000）. DcPP2C24 was significantly up-regulated by high temperature（42 ℃）. And DcPP2C5 was up-regulated by low temperature（4 ℃）. DcPPC20 was induced by salt stress（200 mmol · L^-1 NaCl）, and the expression of these genes were all induced by 100 μmol · L^-1ABA and 20 μmol · L^-1 SA to some degrees,suggesting that they played roles in response to the stresses in D. catenatum.

Key words: Dendrobium catenatum, protein phosphatase 2C, abiotic stress, plant growth regulator, expression analysis

CLC Number:

S567

ZHANG Tingting, LI Yuxin, ZHANG Deyao, KANG Yuqian, WANG Jian, SONG Xiqiang, ZHOU Yang. Genome-Wide Identification and Expression Analyses of PP2C Gene Family in Dendrobium catenatum[J]. Acta Horticulturae Sinica, 2021, 48(12): 2458-2470.

Figures/Tables 7

References 36

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基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
ACTIN	TTGTGTTGGATTCTGGTGATGGTGT	TTTCCCGTTCTGCTGTTGTTGTGAA
DcPP2C5	CTTTGAAGATGTTGAGTTCCCAC	TGCTTTCCGCACTGAATCCT
DcPP2C6	ATCACTGTTGTGGACCTTTGCG	CGCACACGGCAGAGAGGCTA
DcPP2C16	AGGGCAAACTTCTGGGACTACTG	AGGGCAAACTTCTGGGACTACTG
DcPP2C20	GTGGGAAAAAATGTGAGAAAGAGT	TTGGGTCCATCTCCAGTATTCTT
DcPP2C24	CCTGAGGACTCTTTAGCCATTGC	GTGATGTAAGGTGAGGTTCCAATGT
DcPP2C29	TTCAAGGCAGGTATGGGCGTA	TCGCATCATTCATCTGCTCCA
DcPP2C35	CTTCAGGAACGACAGTTACATTTG	TCCAACCTCACCCCCACTT
DcPP2C38	CACCTGGGAGTGACGACGAT	CCAGACATACCAAGTTCAAATCCC
DcPP2C56	CTTGGCAACCGTTTATTTGTAGC	CACATTACTACTCCTCCCGCATC
DcPP2C62	CATTGTTGAGGGGCAAGAGAG	GCACCACTCATCATCAACGC

基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
ACTIN	TTGTGTTGGATTCTGGTGATGGTGT	TTTCCCGTTCTGCTGTTGTTGTGAA
DcPP2C5	CTTTGAAGATGTTGAGTTCCCAC	TGCTTTCCGCACTGAATCCT
DcPP2C6	ATCACTGTTGTGGACCTTTGCG	CGCACACGGCAGAGAGGCTA
DcPP2C16	AGGGCAAACTTCTGGGACTACTG	AGGGCAAACTTCTGGGACTACTG
DcPP2C20	GTGGGAAAAAATGTGAGAAAGAGT	TTGGGTCCATCTCCAGTATTCTT
DcPP2C24	CCTGAGGACTCTTTAGCCATTGC	GTGATGTAAGGTGAGGTTCCAATGT
DcPP2C29	TTCAAGGCAGGTATGGGCGTA	TCGCATCATTCATCTGCTCCA
DcPP2C35	CTTCAGGAACGACAGTTACATTTG	TCCAACCTCACCCCCACTT
DcPP2C38	CACCTGGGAGTGACGACGAT	CCAGACATACCAAGTTCAAATCCC
DcPP2C56	CTTGGCAACCGTTTATTTGTAGC	CACATTACTACTCCTCCCGCATC
DcPP2C62	CATTGTTGAGGGGCAAGAGAG	GCACCACTCATCATCAACGC

基因名称 Gene name	基因 ID Gene ID	基因位置 Genome location	氨基酸/aa Amino acid	分子量/kD Molecular weight	等电点 Isoelectric point
DcPP2C1	LOC110092834	NW_021319682.1 12353861…12363554	429	46.53	4.79
DcPP2C2	LOC110093201	NW_021319838.1 79636…86716	357	38.54	5.92
DcPP2C3	LOC110097041	NW_021445045.1 22930…89839	376	41.44	5.73
DcPP2C4	LOC110092981	NW_021479630.1 566050…569127	437	48.44	5.73
DcPP2C5	LOC110106882	NW_021319908.1 1599017…1626996	656	73.43	6.05
DcPP2C6	LOC110093401	NW_021319500.1 2605723…2631661	355	39.03	5.44
DcPP2C7	LOC110096102	NW_021318918.1 327845…329678	349	38.17	5.57
DcPP2C8	LOC110092255	NW_021319550.1 589338…598830	289	31.62	6.90
DcPP2C9	LOC110102146	NW_021318673.1 106681…130525	283	31.37	6.13
DcPP2C10	LOC110101792	NW_021437127.1 239…864	169	18.38	5.19
DcPP2C11	LOC110116114	NW_021319083.1 7218367…7229170	363	39.77	5.22
DcPP2C12	LOC110103211	NW_021319536.1 1067495…1070936	342	37.17	5.22
DcPP2C13	LOC110108169	NW_021320036.1 164362…168605	403	44.84	6.30
DcPP2C14	LOC110101987	NW_021319486.1 1718938…1721588	394	43.43	4.77
DcPP2C15	LOC110097073	NW_021319809.1 112607…115681	404	44.61	5.01
DcPP2C16	LOC110094890	NW_021319875.1 884026…895795	433	46.69	8.12
DcPP2C17	LOC110101160	NW_021320015.1 317054…320264	463	51.24	5.47
DcPP2C18	LOC110103650	NW_021319857.1 597472…599426	521	58.42	5.78
DcPP2C19	LOC110107612	NW_021319184.1 52038…60613	536	57.64	4.97
DcPP2C20	LOC110092282	NW_021319843.1 1140274…1141088	377	40.61	5.67
DcPP2C21	LOC110092686	NW_021318775.1 1151130…1158463	391	43.31	6.47
DcPP2C22	LOC110100546	NW_021542761.1 641641…655285	306	33.64	6.32
DcPP2C23	LOC110116041	NW_021369505.1 23106…26698	217	23.13	5.31
DcPP2C24	LOC110105480	NW_021319083.1 17250298…17253552	645	71.49	5.58
DcPP2C25	LOC110093956	NW_021319004.1 5426598…5430388	597	65.52	5.66
DcPP2C26	LOC110111364	NW_021319303.1 345392…366805	432	46.00	5.27
DcPP2C27	LOC110095027	NW_021320057.1 2721345…2737862	399	43.97	8.90
DcPP2C28	LOC110116281	NW_021319063.1 1121923…1141845	376	42.47	6.60
DcPP2C29	LOC110111869	NW_021319891.1 154900…158247	387	42.31	5.57
DcPP2C30	LOC110111777	NW_021319083.1 28116466…28125329	285	31.08	6.18
DcPP2C31	LOC110102478	NW_021319127.1 3797515…3799946	408	44.11	5.81
DcPP2C32	LOC110110966	NW_021318758.1 3751…4653	270	29.36	5.99
DcPP2C33	LOC110092340	NW_021319405.1 21754…29592	613	67.67	5.92
DcPP2C34	LOC110098931	NW_021319048.1 1082370…1091816	440	47.25	5.98
DcPP2C35	LOC110114396	NW_021319770.1 96534…111295	445	48.21	6.28
DcPP2C36	LOC110115466	NW_021319945.1 1826754…1859103	438	47.35	8.10
DcPP2C37	LOC110101385	NW_021319178.1 7021686…7023466	408	44.08	6.47
DcPP2C38	LOC110109810	NW_021319281.1 570006…587525	564	60.50	4.75
DcPP2C39	LOC110102541	NW_021319394.1 554440…558932	366	40.43	8.14
DcPP2C40	LOC110113698	NW_021319086.1 1277580…1305129	843	93.73	5.15
DcPP2C41	LOC110110144	NW_021319483.1 1927209…1943550	294	31.93	4.82
DcPP2C42	LOC110113512	NW_021320183.1 343309…347253	405	44.74	9.13
DcPP2C43	LOC110109375	NW_021318890.1 6027…43817	369	40.74	6.73
DcPP2C44	LOC110114968	NW_021540301.1 77…542	80	8.86	4.13
DcPP2C45	LOC110103345	NW_021319127.1 148186…155682	379	41.74	8.38
DcPP2C46	LOC110099265	NW_021319673.1 21670…23848	386	42.73	5.75
DcPP2C47	LOC110095534	NW_021319289.1 235158…239974	361	40.80	6.16
DcPP2C48	LOC110096184	NW_021320128.1 576030…582730	382	43.03	6.90
DcPP2C49	LOC110102425	NW_021319836.1 605740…611552	364	40.36	8.09
DcPP2C50	LOC110094144	NW_021319833.1 29275…42783	332	36.68	5.63
DcPP2C51	LOC110098988	NW_021387661.1 202…910	136	14.80	6.13
DcPP2C52	LOC110104643	NW_021319518.1 7586364…7608658	963	106.49	5.56
DcPP2C53	LOC110107952	NW_021318744.1 279285…290373	956	106.29	6.10
DcPP2C54	LOC110101653	NW_021319798.1 157820…165707	351	38.27	5.27
基因名称 Gene name	基因 ID Gene ID	基因位置 Genome location	氨基酸/aa Amino acid	分子量/kD Molecular weight	等电点 Isoelectric point
DcPP2C55	LOC110104152	NW_021320112.1 176838…213807	1 075	119.59	5.12
DcPP2C56	LOC110094641	NW_021319416.1 6703…36842	322	36.03	8.71
DcPP2C57	LOC110113113	NW_021319434.1 271374…304456	356	39.15	5.21
DcPP2C58	LOC110105369	NW_021319475.1 45167…49612	285	31.31	8.28
DcPP2C59	LOC110102674	NW_021318663.1 311998…323025	216	23.05	5.94
DcPP2C60	LOC110106056	NW_021319959.1 659303…671684	392	43.74	7.70
DcPP2C61	LOC110108569	NW_021319385.1 917516…937291	480	52.41	5.89
DcPP2C62	LOC110099044	NW_021320094.1 1386063…1409016	512	56.12	5.32
DcPP2C63	LOC110092171	NW_021319127.1 3301272…3324232	625	69.12	6.31
DcPP2C64	LOC110098867	NW_021319985.1 222186…229368	384	42.55	9.36
DcPP2C65	LOC110108709	NW_021318852.1 2065548…2077099	289	30.98	4.77
DcPP2C66	LOC110112047	NW_021318824.1 229329…231733	340	37.92	7.03
DcPP2C67	LOC110115916	NW_021319683.1 571454…602635	380	41.94	6.16

基因名称 Gene name	基因 ID Gene ID	基因位置 Genome location	氨基酸/aa Amino acid	分子量/kD Molecular weight	等电点 Isoelectric point
DcPP2C1	LOC110092834	NW_021319682.1 12353861…12363554	429	46.53	4.79
DcPP2C2	LOC110093201	NW_021319838.1 79636…86716	357	38.54	5.92
DcPP2C3	LOC110097041	NW_021445045.1 22930…89839	376	41.44	5.73
DcPP2C4	LOC110092981	NW_021479630.1 566050…569127	437	48.44	5.73
DcPP2C5	LOC110106882	NW_021319908.1 1599017…1626996	656	73.43	6.05
DcPP2C6	LOC110093401	NW_021319500.1 2605723…2631661	355	39.03	5.44
DcPP2C7	LOC110096102	NW_021318918.1 327845…329678	349	38.17	5.57
DcPP2C8	LOC110092255	NW_021319550.1 589338…598830	289	31.62	6.90
DcPP2C9	LOC110102146	NW_021318673.1 106681…130525	283	31.37	6.13
DcPP2C10	LOC110101792	NW_021437127.1 239…864	169	18.38	5.19
DcPP2C11	LOC110116114	NW_021319083.1 7218367…7229170	363	39.77	5.22
DcPP2C12	LOC110103211	NW_021319536.1 1067495…1070936	342	37.17	5.22
DcPP2C13	LOC110108169	NW_021320036.1 164362…168605	403	44.84	6.30
DcPP2C14	LOC110101987	NW_021319486.1 1718938…1721588	394	43.43	4.77
DcPP2C15	LOC110097073	NW_021319809.1 112607…115681	404	44.61	5.01
DcPP2C16	LOC110094890	NW_021319875.1 884026…895795	433	46.69	8.12
DcPP2C17	LOC110101160	NW_021320015.1 317054…320264	463	51.24	5.47
DcPP2C18	LOC110103650	NW_021319857.1 597472…599426	521	58.42	5.78
DcPP2C19	LOC110107612	NW_021319184.1 52038…60613	536	57.64	4.97
DcPP2C20	LOC110092282	NW_021319843.1 1140274…1141088	377	40.61	5.67
DcPP2C21	LOC110092686	NW_021318775.1 1151130…1158463	391	43.31	6.47
DcPP2C22	LOC110100546	NW_021542761.1 641641…655285	306	33.64	6.32
DcPP2C23	LOC110116041	NW_021369505.1 23106…26698	217	23.13	5.31
DcPP2C24	LOC110105480	NW_021319083.1 17250298…17253552	645	71.49	5.58
DcPP2C25	LOC110093956	NW_021319004.1 5426598…5430388	597	65.52	5.66
DcPP2C26	LOC110111364	NW_021319303.1 345392…366805	432	46.00	5.27
DcPP2C27	LOC110095027	NW_021320057.1 2721345…2737862	399	43.97	8.90
DcPP2C28	LOC110116281	NW_021319063.1 1121923…1141845	376	42.47	6.60
DcPP2C29	LOC110111869	NW_021319891.1 154900…158247	387	42.31	5.57
DcPP2C30	LOC110111777	NW_021319083.1 28116466…28125329	285	31.08	6.18
DcPP2C31	LOC110102478	NW_021319127.1 3797515…3799946	408	44.11	5.81
DcPP2C32	LOC110110966	NW_021318758.1 3751…4653	270	29.36	5.99
DcPP2C33	LOC110092340	NW_021319405.1 21754…29592	613	67.67	5.92
DcPP2C34	LOC110098931	NW_021319048.1 1082370…1091816	440	47.25	5.98
DcPP2C35	LOC110114396	NW_021319770.1 96534…111295	445	48.21	6.28
DcPP2C36	LOC110115466	NW_021319945.1 1826754…1859103	438	47.35	8.10
DcPP2C37	LOC110101385	NW_021319178.1 7021686…7023466	408	44.08	6.47
DcPP2C38	LOC110109810	NW_021319281.1 570006…587525	564	60.50	4.75
DcPP2C39	LOC110102541	NW_021319394.1 554440…558932	366	40.43	8.14
DcPP2C40	LOC110113698	NW_021319086.1 1277580…1305129	843	93.73	5.15
DcPP2C41	LOC110110144	NW_021319483.1 1927209…1943550	294	31.93	4.82
DcPP2C42	LOC110113512	NW_021320183.1 343309…347253	405	44.74	9.13
DcPP2C43	LOC110109375	NW_021318890.1 6027…43817	369	40.74	6.73
DcPP2C44	LOC110114968	NW_021540301.1 77…542	80	8.86	4.13
DcPP2C45	LOC110103345	NW_021319127.1 148186…155682	379	41.74	8.38
DcPP2C46	LOC110099265	NW_021319673.1 21670…23848	386	42.73	5.75
DcPP2C47	LOC110095534	NW_021319289.1 235158…239974	361	40.80	6.16
DcPP2C48	LOC110096184	NW_021320128.1 576030…582730	382	43.03	6.90
DcPP2C49	LOC110102425	NW_021319836.1 605740…611552	364	40.36	8.09
DcPP2C50	LOC110094144	NW_021319833.1 29275…42783	332	36.68	5.63
DcPP2C51	LOC110098988	NW_021387661.1 202…910	136	14.80	6.13
DcPP2C52	LOC110104643	NW_021319518.1 7586364…7608658	963	106.49	5.56
DcPP2C53	LOC110107952	NW_021318744.1 279285…290373	956	106.29	6.10
DcPP2C54	LOC110101653	NW_021319798.1 157820…165707	351	38.27	5.27
基因名称 Gene name	基因 ID Gene ID	基因位置 Genome location	氨基酸/aa Amino acid	分子量/kD Molecular weight	等电点 Isoelectric point
DcPP2C55	LOC110104152	NW_021320112.1 176838…213807	1 075	119.59	5.12
DcPP2C56	LOC110094641	NW_021319416.1 6703…36842	322	36.03	8.71
DcPP2C57	LOC110113113	NW_021319434.1 271374…304456	356	39.15	5.21
DcPP2C58	LOC110105369	NW_021319475.1 45167…49612	285	31.31	8.28
DcPP2C59	LOC110102674	NW_021318663.1 311998…323025	216	23.05	5.94
DcPP2C60	LOC110106056	NW_021319959.1 659303…671684	392	43.74	7.70
DcPP2C61	LOC110108569	NW_021319385.1 917516…937291	480	52.41	5.89
DcPP2C62	LOC110099044	NW_021320094.1 1386063…1409016	512	56.12	5.32
DcPP2C63	LOC110092171	NW_021319127.1 3301272…3324232	625	69.12	6.31
DcPP2C64	LOC110098867	NW_021319985.1 222186…229368	384	42.55	9.36
DcPP2C65	LOC110108709	NW_021318852.1 2065548…2077099	289	30.98	4.77
DcPP2C66	LOC110112047	NW_021318824.1 229329…231733	340	37.92	7.03
DcPP2C67	LOC110115916	NW_021319683.1 571454…602635	380	41.94	6.16

Genome-Wide Identification and Expression Analyses of PP2C Gene Family in Dendrobium catenatum

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