%0 Journal Article %A Shasha LI %A Saisai YU %A Yuheng FU %A Qiangwei LUO %A Yan XU %A Yuejin WANG %T The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes %D 2022 %R 10.16420/j.issn.0513-353x.2021-0141 %J Acta Horticulturae Sinica %P 723-738 %V 49 %N 4 %X

New germplasms of seedless and cold-resistant grapes were cultivated by embryo rescue techniques after crossing in the field and by molecular marker-assisted selection using stenospermocarpic seedless grapes as the female parents and varieties of Chinese cold-resistant wild species or hybrids from Vitis vinifera × V. amurensis as the male parents. Among the 14 hybrid combinations,a total of 1 021 hybrid plantlets were obtained,among which 634 plantlets were obtained in the combination of‘Ruby Seedless’בBeibinghong’with the highest seedling rate of 18.13%. When‘Zuoyouhong’was the male parent,‘Flame Seedless’was suitable as female parent. When‘Beibinghong’and‘Xuelanhong’were as the male parent,‘Ruby Seedless’was suitable as female parent. When‘Beichun’as the male parent,‘Kunxiang Seedless’was suitable as female parent. A total of 132 lines related to seedlessness preliminary screened by the two seedless molecular markers SCF27 and GSLP1. A total of 190 lines related to cold-resistant were initially screened through the molecular marker S241-717. Among these hybrid progeny screened,59 lines possessed both seedless and cold-resistant traits. The grapevine cold-related genes CBF1,COR27 and ERD15 detected by qRT-PCR in the lines with or without cold-resistant traits of the hybrid progeny of‘Ruby Seedless’בBeibinghong’and‘Ruby Seedless’בXuelanhong’under cold treatment were differential expression,and the cold-related lines showed significant expression of the cold-resistant genes.

%U https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-0141