ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (1): 127-142.doi: 10.16420/j.issn.0513-353x.2019-0219

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Identification and Expression Analysis of CIPK Gene Family in Strawberry

LIU Tao,WANG Pingping,HE Honghong,LIANG Guoping,LU Shixiong,CHEN Baihong,and MAO Juan*   

  1. College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China
  • Online:2020-01-25 Published:2020-01-25

Abstract: The Arabidopsis CIPK gene family registration numbers were obtained from the Database,and 19 CIPK gene family members were obtained from the Fragaria vesca database by bioinformatics analysis,which were divided into six subfamilies:A–E. The six subfamilies were distributed on 6 of the 7 chromosomes of strawberry. By analyzing physicochemical properties,we found that the number of encoded amino acids is ranged from 157 to 1 196,the theoretical isoelectric point is covered from 3.91 to 9.34 and the molecular weight is distributed from 18 667.68 to 133 714.31 D. Meanwhile,there are 11 genes that have only one exon,the others have 2–15 exons through genes structure analysis. The CIPK gene family were mainly expressed on the cytoplasm,nucleus and chloroplast during subcellular localization analysis of the gene family members. Moreover,the prediction of secondary structure indicated that the members of the gene family mainly consisted of α-helix,β-turn and irregular curl. Additionally,analysis of the cis-acting element of the upstream 2 kb promoter sequence found that the members of the gene family contained stress response MYB element. Except for FvCIPK02,FvCIPK15 and FvCIPK17,all genes contained the abscisic acid(ABA)response element(ABRE). The qRT-PCR analysis showed that the FvCIPK16,FvCIPK10 and FvCIPK09 had the highest expression under PEG,ABA and NaCl,respectively,about 18.4 times,29 times and 13 times more than the control,which indicated that FvCIPK16 responded strongly to drought stress,FvCIPK10 strongly responded to ABA induction,and FvCIPK09 strongly responded to high salt stress. In addition,the relative expression of FvCIPK03 was down-regulated under each treatment,then we can speculate that FvCIPK03 play a negative regulatory role in plant stress.

Key words: strawberry, CIPK gene family, qRT-PCR, bioinformatics analysis.

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