Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 456-464.doi: 10.16420/j.issn.0513-353x.2020-0499

• Research Papers • Previous Articles     Next Articles

Inhibition of Pear Anthocyanin Synthesis by the PcHB12 Gene in European Pears

ZHANG Zhen, LI Chenzhiyu, ZHANG Ya, LI Xi, WANG Shuo, WANG Xinyu, CHEN Xuesen, FENG Shouqian()   

  1. College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Tai'an,Shandong 271018,China
  • Received:2020-08-01 Online:2021-03-25 Published:2021-04-02
  • Contact: FENG Shouqian


In order to understand the mechanism that the HD-ZipⅠtranscription factor regulates anthocyanin synthesis in pears,the European pear cultivar‘Red Clapp Favorite’was used as study material to clonePcHB12(GenBank accession number:XM_009363028)from the HD-ZipⅠfamily. Phylogenetic tree analysis,qRT-PCR,yeast one-hybrid assay,EMSA,and luciferase reporter assay were employed to study the regulatory function ofPcHB12on anthocyanin synthesis. The results showed that the open reading frame of PcHB12 is 696 bp,encoding 231 amino acids,and the predicted molecular weight of the protein is 26.77 kD. Phylogenetic tree analysis showed that PcHB12 has the highest similarity to the AtHB12 protein sequence of Arabidopsis thaliana. The expression level of PcHB12 in‘Clapp Favorite’is significantly higher than that in its red bud mutation cultivar‘Red Clapp Favorite’. In contrast,anthocyanin content and the expression levels of anthocyanin-related genesPcMYB10.1 and PcUFGT in ‘Clapp Favorite’were significantly lower than that in‘Red Clapp Favorite’. Yeast one-hybrid and electrophoretic mobility shift assay(EMSA)experiments found that PcHB12 binds to the MBS sequence in the PcMYB10.1 promoter. Luciferase experiments showed that PcHB12 negatively regulates transcription activity on the PcMYB10.1 promoter. Therefore,PcHB12 may negatively regulate PcMYB10.1 expression to inhibit anthocyanin synthesis in pears.

Key words: pear, anthocyanin, PcHB12, PcMYB10.1

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