Research on the Response Mechanism of Lignin in Carrot Taproot Under Salt Stress

doi:10.16420/j.issn.0513-353x.2020-0139

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (1): 153-161.doi: 10.16420/j.issn.0513-353x.2020-0139

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Research on the Response Mechanism of Lignin in Carrot Taproot Under Salt Stress

CHEN Haowei¹, CHEN Mengjiao¹, WANG Yahui¹, ZHANG Rongrong¹, WANG Xinrui¹, XU Zhisheng¹, TAN Guofei², XIONG Aisheng¹^,^*()

¹College of Horticulture,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Nanjing Agricultural University,Nanjing 210095,China
²Institute of Horticulture,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China

Received:2020-07-17 Revised:2020-09-08 Online:2021-01-25 Published:2021-01-29
Contact: XIONG Aisheng E-mail:xiongaisheng@njau.edu.cn

Abstract

Abstract:

‘Hongfu Qicun’and‘Hongxin Qicun’carrots were used as materials to detect the lignin contents in taproot. Stress-related physiological parameters in taproots and leaves under different NaCl concentration treatments were also determined. The expression levels of 13 lignin biosynthesis related genes in taproot were detected by real-time fluorescence quantitative PCR method. The results showed that the lignin contents of carrot taproot under different salt stress treatments were significantly higher than that of the control（distilled water treatment）. The lignin contents were the highest under 100 mmol·L^-1 NaCl treatment,which were 1.91 times（‘Hongfu Qicun’）and 1.83 times（‘Hongxin Qicun’）of the control,respectively. The results indicated that salt stress induced the biosynthesis of lignin in carrot taproot. The qRT-PCR and correlation analysis results showed that the DcF5H and DcCOMT expression levels of 13 lignin-related genes were similar and high-positively correlated with the dynamic changes of lignin content. The two genes may be the key lignin biosynthesis related genes of carrot taproot in response to salt stress. The physiological indexes demonstrated that the superoxide dismutase（SOD）activity,proline and soluble protein contents in carrot taproots and leaves responded to salt stress treatments. The results of this study indicated that salt stress induced the lignification of carrot taproot,and lignin biosynthesis pathway genes were involved in response to salt stress. DcF5H and DcCOMT may be the key regulating genes of lignin biosynthesis under salt stress in carrot taproot.

Key words: carrot, salt stress, lignin, gene expression, physiological characteristics

CLC Number:

S631.2

CHEN Haowei, CHEN Mengjiao, WANG Yahui, ZHANG Rongrong, WANG Xinrui, XU Zhisheng, TAN Guofei, XIONG Aisheng. Research on the Response Mechanism of Lignin in Carrot Taproot Under Salt Stress[J]. Acta Horticulturae Sinica, 2021, 48(1): 153-161.

Figures/Tables 4

Fig. 1 Changes of‘Hongfu Qicun’and‘Hongxin Qicun’carrots under salt stress treatments The different lowercases indicate significant differences at 0.05 level. The same below.

Fig. 2 Lignin contents of‘Hongfu Qicun’and‘Hongxin Qicun’carrot taproots under salt stress treatments

Fig. 3 Relative expression levels of lignin biosynthesis related genes in‘Hongfu Qicun’and‘Hongxin Qicun’carrot taproots under salt stress treatments

Table 1 Correlation coefficients of taproots lignin contents and lignin biosynthesis related genes’ relative expression levels under salt stress treatments

基因	相关系数	基因	相关系数
Gene	Correlation coefficient	Gene	Correlation coefficient
DcPAL	0.175	DcCOMT	0.955^**
DcC4H	-0.277	DcCCR	-0.015
Dc4CL	0.478^*	DcCAD	-0.296
DcHCT	-0.474^*	DcPER1	-0.696^**
DcC3′H	-0.270	DcLAC1	-0.760^**
DcCCoAOMT	-0.475^*	DcLAC2	-0.289
DcF5H	0.902^**

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Research on the Response Mechanism of Lignin in Carrot Taproot Under Salt Stress

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