Abstract: ‘Kyoho’grapevine cultured under root restriction for 3 years were studied for the various character of contents of carbohydrates and nitrogen compounds with the vines cultured with traditional methods as the control. Contents of physiological indexs such as sugar，starch，total nitrogen and amino acids in different vegetative organs were investigated at veraison and harvest stage. The results showed that compared with the control，shoot length and leaf areas were reduced significantly under root restriction，whereas the soluble solids content in berry was higher than that in the control. Root restricted grapevines had the higher sugar and starch contents in the root，trunk and shoots than the control，however，the content of total amino acids under root restriction was only half of that in the control. In addition，content of the total nitrogen，NO3--N，NH4+-N and all amino acid content except arginine，were lower than that in the control. Therefore，it is suggested that the nitrogen uptake and amino acid synthesisin xylem sap were inhibited by root restriction at veraison and harvest stage，which might lead to accelerate more photosynthetic products shipped to fruits and promote the accumulation of sugar in berries.

Key words: grape, root zone restriction, carbohydrate, nitrogen content, amino acid