Abstract:

Using the cDNA sequences from Hyper variable（HV）regions of 52 S-alleles in Oriental pear cultivars，cDNA microarray for S-RNase detections was established. Totally 240 dots and 55 cDNA probes，which includes all the HV specific cDNA sequences of perfected S-RNase gene，were spotted on the chip. By Cy3-labeled primers and cDNA template of tested cultivars pistils，the Cy3-labeled specific cDNA PCR products of S-RNase were amplified and hybridized with the cDNA microarray in order to detect the S genotype of pear cultivars. Cultivars with known S-genotype such as Lijiang Huangsuanli，Xiuyu，Midu Yuli，Baimianli and Deshengxiang were S-genotyped using the cDNA microarray and the results showed that the microarray analyses were consistent with their oligonucleotide genechip analyses and their RFLP and DNA sequenced results. Then the S-RNase cDNA microarrays and the oligonucleotide genechips were used to parallel S-genotyping 24 sand pear cultivars with unknown S-genotype and their S-genotypes were determined. In conclusion，the construction of cDNA microarrays has further improved the pear S-RNase detection platform.

Key words: pear, self-incompatibility, S-genotype, cDNA microarray, oligonucleotide genechip