Preliminary Investigation on Sex Differentiation of Actinidia chinensis by High-throughput microRNAs Sequencing

doi:10.16420/j.issn.0513-353x.2015-0166

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (7): 1260-1272.doi: 10.16420/j.issn.0513-353x.2015-0166

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Preliminary Investigation on Sex Differentiation of Actinidia chinensis by High-throughput microRNAs Sequencing

YAN Ming-Ke, XU Qiang, LIU Chun-Yan, ZHANG Qiong, YAO Xiao-Hong

1Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture，Wuhan Botanical Garden，Chinese Academy of Sciences，Wuhan 430074，China；2University of Chinese Academy of Sciences，Beijing 100049，China；3Shangrao Vocational & Technical College，Shangrao，Jiangxi 334109，China

Online:2015-07-25 Published:2015-07-25

Abstract

Abstract: The miRNAs in female and male flowers of kiwifruit were sequenced by next-generation sequencing，and received 18 408 610 and 11 191 469 reads，respectively. Using bioinformatics methods，39 conserved and 400 novel miRNA families including 170 miRNA families that were differently expressed between the two samples were identified. Targets prediction and annotation of identified miRNAs revealed the most enrichment of P-loop containing nucleoside triphosphate hydrolase domain in InterPro terms. Three miRNAs were predicted to be located on the kiwifruit sex chromosome（Chr25），among which，target gene Achn298021 of novel-ach-miR362 was predicted to be related to flower development，and should be drawn special attention in future study.

Key words: kiwifruit, microRNA, high-throughput sequencing, sex determination

CLC Number:

S 663.4

YAN Ming-Ke, XU Qiang, LIU Chun-Yan, ZHANG Qiong, YAO Xiao-Hong. Preliminary Investigation on Sex Differentiation of Actinidia chinensis by High-throughput microRNAs Sequencing[J]. ACTA HORTICULTURAE SINICA, 2015, 42(7): 1260-1272.

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Preliminary Investigation on Sex Differentiation of Actinidia chinensis by High-throughput microRNAs Sequencing

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