Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 23-40.doi: 10.16420/j.issn.0513-353x.2020-0969

• Research Papers • Previous Articles     Next Articles

Identification and Expression Analysis of MicroRNA Families Associated with Phase Transition in Chinese Jujube

LI Ying1,2, MENG Xianwei1,2, MA Zhihang2, LIU Mengjun1,2,**(), ZHAO Jin3,**()   

  1. 1Research Center of Chinese Jujube,Hebei Agricultural University,Baoding,Hebei 071000,China
    2College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China
    3College of Life Science,Hebei Agricultural University,Baoding,Hebei 071000,China
  • Received:2021-04-06 Revised:2021-05-26 Online:2022-01-25 Published:2022-01-24
  • Contact: LIU Mengjun,ZHAO Jin;


Current studies have shown that a number of microRNA(miRNA)families are involved in plant flowering time regulation and phase transition,etc. Therefore,it is very instructive for the phase transition regulation of fruit trees to gain insight into microRNA family associated with phase transition in jujube tree. In this study,the new shoots at different development stages(nodes)of jujube progenies were used as materials. Firstly,27 miRNA families with 58 known miRNAs and 23 novel miRNAs were identified by small RNA sequencing analysis in the three different development stages of juvenile,transition and adult period. Among them,44 miRNAs were differentially expressed,including 32 known miRNAs and 12 novel miRNAs. Afterward,six miRNAs(zjmiR156a-5p,zjmiR156j,zjmiR172c,zjmiRmir172e-3p,zjmiRnovel-16 and zjmiRnovel-71)with obviously differential expression in the three development stages were screened out. Their 15 key target genes were also predicted through TargetFinder software,and they are mainly involved in flowering,transcriptional regulation and biosynthesis. Finally,the differential expression of the above-mentioned 12 miRNAs and their 15 key target genes during phase transition were verified by qRT-PCR,and they had positive or negative regulatory relationships. Moreover,the two newly discovered miRNAs(zjmiRnovel-16 and zjmiRnovel-71)may affect the phase transition through photoperiod pathway and glucose metabolism pathway,respectively.

Key words: Ziziphus jujuba, phase transition, microRNA, target gene, small RNA sequencing

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