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ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (2): 214-220.doi: 10.16420/j.issn.0513-353x.2014-0871

• Fruit Trees • Previous Articles     Next Articles

Screening of SNP Markers Tightly Linked to PcDw Locus Determining Pear Dwarf Trait Using HRM Technology

LI Wei,TIAN Yi-ke,WANG Cai-hong*,BAI Mu-dan,and HOU Dong-liang   

  1. College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China
  • Online:2015-02-25 Published:2015-02-25

Abstract: Screening of SNP markers tightly linked to objective traits is critical for fine mapping of objective gene due to abundant SNP loci exist in the genome of fruits. In this research,26 primer pairs which are suit for high resolution melting(HRM)analysis were designed according to the sequences from apple genome. Based on the principle of the bulked segregant analysis(BSA),these primer pairs were tested in the F1 population with 215 individuals derived from the cross of Pyrus communis L.‘Aishengli’× Pyrus bretschneideri Rehd.‘Chili’by HRM,and two markers(CNqau012 and CNqau039)linked to PcDw locus at the distance of 13.3 cM and 2.33 cM,respectively,were identified. Furthermore,the amplicons of the two markers(CNqau012 and CNqau039)were sequenced and one single nucleotide changes(G/A and C/T,respectively)between the dwarf and standard phenotypes were detected. These SNP markers are of great significance to the following fine mapping and cloning of the PcDw gene.

Key words: pear, dwarf, PcDw, SNP marker, HRM

CLC Number: