Abstract: Effects of Chaetomium globosum ND35 fungal fertilizer on the replanted soil environment and Malus hupehensis Rehd. seedlings biomass were investigated in pot experiments. The experiment involved three treatments，including a control，the fungus chaff fertilizered into the soil which is carrierfungus chaff of Chaetomium globosum and Chaetomium globosum ND35 fungal fertilizer，a mixture of fungus chaff and strains，fertilizered into the soil with the concentration of 2%. Results showed that the fungus chaff and Chaetomium globosum ND35 fungal fertilizer application to replant soil significantly enhanced the dry and fresh weight of the seedlings compared with the control. And Chaetomium globosum ND35 fungal fertilizer increased in dry and fresh weight by 71.9% and 71.6% in July，155.9% and 153.1% in September than the control，respectively. The effects are more obvious in September. As the extension of the treating time，the quantities of soil bacteria and actinomycete were increased，and reached peak in September. Chaetomium globosum ND35 fungal fertilizer had a stronger effect. Compared with control，the quantity of soil bacteria in July，August and September increased by 245.9%，89.1% and 216.3%，respectively. The quantity of soil actinomycete increased by 68.7%，124.7% and 131.1% respectively. Both of the two treatments increased the activity of soil enzymes which increased with a subsequent decrease initially，and was the highest in August. The fungal fertilizer showed a significant increase to soil enzymes. The fungal fertilizer fertilizered into the soil significantly improved the root activity of Malus hupehensis Rehd.，and increased by 59.0%，50.2% and 20.2%，respectively in July，August and September. Compared with control and the fungus chaff，Chaetomium globosum ND35 fungal fertilizer can improve soil microflora，improve soil enzyme activity，increase root activity and biomass of Malus hupehensis Rehd. seedlings.

Key words: Malus hupehensis, endophytic fungi, Chaetomium globosum ND35, organic fertilizer, replant, soil environment